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Killian's is it a True Dehiscence? An Anatomical Perspective.
Cureus
September 2020
Otolaryngology - Head and Neck Surgery, Charlotte Maxeke Johannesburg Academic Hospital, Johannesburg, ZAF.
Objective Zenker's diverticulum is a pulsion outpouching from the posterior pharyngeal wall. The anatomy of the wall has been proposed to be dehiscent in the region of the Killian's triangle, between the thyropharyngeus muscle (inferior pharyngeal constrictor) and the cricopharyngeus muscle. A dehiscence is a bursting open, splitting or gaping along natural or sutured lines.
View Article and Find Full Text PDFThe importance of fixation procedures on DNA template and its suitability for solution-phase polymerase chain reaction and PCR in situ hybridization.
Histochem J
April 1994
Department of Pathology, University of Leeds, UK.
Conventional solution-phase polymerase chain reaction (PCR) and in situ PCR/PCR in situ hybridization are powerful tools for retrospective analysis of fixed paraffin wax-embedded material. Amplification failure using these techniques is now encountered in some centres using archival fixed tissues. Such 'failures' may not only be due to absent target DNA sequences in the tissues, but may be a direct effect of the type of fixative, fixation time and/or fixation temperature used.
View Article and Find Full Text PDFUse of monoclonal anti-actin as a megakaryocyte marker in paraffin wax embedded bone marrow biopsy specimens.
J Clin Pathol
September 1989
Servei d'Hematologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Monoclonal anti-actin was used as a marker of megakaryocytes in Zenker's fixed, paraffin wax embedded bone marrow tissue, using an immunoperoxidase staining method. Twenty bone marrow samples were studied, including controls, and different myeloproliferative and myelodysplastic syndromes. The results were compared with those obtained using factor VIII related antigen (F VIII RAg) immunolabelling.
View Article and Find Full Text PDFTen human jejunal biopsy specimens were examined by both immunofluorescence (IF) and immunoperoxidase (IP) methods to compare both plasma cell counts and the distribution of extracellular immunoglobulins. Each specimen was cut into at least two portions, one fixed in 5% formaldehyde in phosphate-buffered saline before being snap-frozen and sectioned on a cryostat for IF, the other being fixed in half-strength Zenker and embedded in paraffin wax by standard methods for IP. Plasma cell counts were comparable in the eight biopsy specimens for which they could be estimated, geometric mean values being IgA 22.
View Article and Find Full Text PDFNine different fixatives (Carnoy's, Susa, Baker's formalin, 5% formalin, 10% formalin, 10% formol saline, Bouin, Zenker, and 2.5% gluta-aldehyde) were compared by two methods. Gelatin-albumin gels were used to study volume changes after fixation and after various stages of subsequent processing.
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