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Unravelling the impact of fat content on the microbial dynamics and spatial distribution of foodborne bacteria in tri-phasic viscoelastic 3D models.
Sci Rep
December 2023
Bioprocess and Biochemical Engineering Group (BioProChem), Department of Chemical and Process Engineering, University of Surrey, Guildford, GU2 7XH, UK.
Article Synopsis
- The study focused on creating advanced in vitro 3D models resembling real food products by enhancing a bi-phasic model (Xanthan Gum and Whey Protein) with varying concentrations of a fat phase (Sunflower Oil).
- The new tri-phasic models were analyzed for their structural and biochemical properties, showing similarities to industrial food products, particularly in terms of microbial growth dynamics of foodborne bacteria.
- Results indicated that higher fat concentrations led to larger bacterial colonies, particularly near the fat phase, emphasizing the need for complex models to better understand bacterial behavior and responses to decontamination.
After a century of nisin research - where are we now?
FEMS Microbiol Rev
May 2023
APC Microbiome Ireland, University College Cork,Western Road, Cork T12 YN60, Ireland.
It is almost a century since nisin was discovered in fermented milk cultures, coincidentally in the same year that penicillin was first described. Over the last 100 years this small, highly modified pentacyclic peptide has not only found success in the food industry as a preservative but has also served as the paradigm for our understanding of the genetic organization, expression, and regulation of genes involved in lantibiotic biosynthesis-one of the few cases of extensive post-translation modification in prokaryotes. Recent developments in understanding the complex biosynthesis of nisin have shed light on the cellular location of the modification and transport machinery and the co-ordinated series of spatio-temporal events required to produce active nisin and provide resistance and immunity.
View Article and Find Full Text PDFThe disease-producing capacity of the opportunistic pathogen Enterococcus faecalis is enhanced by the ability of the bacterium to evade killing by antimicrobial agents. Survival of E. faecalis in the presence of the human antimicrobial enzyme lysozyme is mediated in part by the site 2 metalloprotease Eep; however, a complete model of enterococcal lysozyme resistance has not been elucidated.
View Article and Find Full Text PDFConstruction of a recombinant food-grade Lactococcus lactis expressing P23 protein of Cryptosporidium parvum.
Folia Microbiol (Praha)
August 2022
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Cryptosporidium parvum infects enterocytes in diverse vertebrates, including humans, and causes diarrheal illness. However, no effective drugs are available for this protozoan infection. The P23 protein of C.
View Article and Find Full Text PDFIsolation and Analysis of the Nisin Biosynthesis Complex NisBTC: further Insights into Their Cooperative Action.
mBio
October 2021
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningengrid.4830.f, Groningen, the Netherlands.
Nisin is synthesized by a putative membrane-associated lantibiotic synthetase complex composed of the dehydratase NisB, the cyclase NisC, and the ABC transporter NisT in Lactococcus lactis. Earlier work has demonstrated that NisB and NisT are linked via NisC to form such a complex. Here, we conducted for the first time the isolation of the intact NisBTC complex and NisT-associated subcomplexes from the cytoplasmic membrane by affinity purification.
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