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The aim of this study was to assess the effect of low-dose electron irradiation on morphological features of the wound healing process in rat skin. Surgical wounds were inflicted with a 2.3 x 1.

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The ultrastructural and histophotometric study of elastic and collagen fibers in type II Ehlers-Danlos syndrome and subclinical forms.

Ultrastruct Pathol

November 2000

Dermatology Division, Faculty of Medical Sciences, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil.

Skin disorders in type II Ehlers-Danlos Syndrome (EDS) are characterized by signs of cutaneous hyperdistensibility, skin and vascular fragility, atrophic scars, and articular hypermobility. These features may have less important clinical presentation in the intermediate forms of type II EDS. The authors studied the ultrastructural and quantitative aspects of elastic and collagen fibers in the skin of individuals with subclinical signs of type II of Ehlers-Danlos Syndrome.

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Basing on the data obtained at early autopsies, we compared the structure and metabolism of liver acini in acute blood loss and pulmonary artery thromboembolism. Morpho- and pathogenetic impairment of hepatocytes of various acinic zones was correlated and studied histo-enzymatically.

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Lectin histochemistry combined with glycosidase digestion was used to investigate the presence of glucidic residues and identify the terminal residues and their acceptor sugars in these glands. Quantitative evaluation of lectin-positive sites was made by histophotometrical scanning. Lectin histochemistry demonstrated that the anteromedial portion contains disaccharides sialic acid-D-Gal, sialic acid-D-GalNAc and Fuc-D-Gal, and that the anterolateral portion contains the terminal dimer sialic acid-D-Gal only.

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