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Generation of super-resolution images from barcode-based spatial transcriptomics by deep image prior.
Cell Rep Methods
December 2024
Portrai, Inc., Dongsullagil, 78-18 Jongrogu, Seoul, Republic of Korea; Department of Nuclear Medicine, Seoul National University Hospital, 03080 Seoul, Republic of Korea; Department of Nuclear Medicine, Seoul National University College of Medicine, 03080 Seoul, Republic of Korea. Electronic address:
Spatially resolved transcriptomics (ST) has revolutionized the field of biology by providing a powerful tool for analyzing gene expression in situ. However, current ST methods, particularly barcode-based methods, have limitations in reconstructing high-resolution images from barcodes sparsely distributed in slides. Here, we present SuperST, an algorithm that enables the reconstruction of dense matrices (higher-resolution and non-zero-inflated matrices) from low-resolution ST libraries.
View Article and Find Full Text PDFGreen-synthesized α-FeO-nanoparticles as potent antibacterial, anti-biofilm and anti-virulence agent against pathogenic bacteria.
BMC Microbiol
December 2024
Department of Physics, College of Science, University of Halabja, Halabja, Kurdistan Region, Iraq.
Background: Antimicrobial resistance (AMR) presents a serious threat to health, highlighting the urgent need for more effective antimicrobial agents with innovative mechanisms of action. Nanotechnology offers promising solutions by enabling the creation of nanoparticles (NPs) with antibacterial properties. This study aimed to explore the antibacterial, anti-biofilm, and anti-virulence effects of eco-friendly synthesized α-Fe₂O₃ nanoparticles (α-Fe₂O₃-NPs) against pathogenic bacteria.
View Article and Find Full Text PDFAnalysis of the MODIST Sequence for Selective Proton-Proton Recoupling.
J Phys Chem A
January 2025
Department of NMR based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, Göttingen 37077, Germany.
Theoretical and simulated analyses of selective homonuclear dipolar recoupling sequences serve as primary tools for understanding and determining the robustness of these sequences under various conditions. In this article, we investigate the recently proposed first-order dipolar recoupling sequence known as MODIST (Modest Offset Difference Internuclear Selective Transfer). We evaluate the MODIST transfer efficiency, assessing its dependence on rf-field strengths and the number of simulated spins, extending up to 10 spins.
View Article and Find Full Text PDFEnhanced aerobic bioremediation of an aquifer heavily contaminated with a mixture of chlorobenzenes and hexachlorocyclohexanes at the Sardas landfill (Spain).
J Hazard Mater
November 2024
Departament d'Enginyeria Química, Biològica i Ambiental, Universitat Autònoma de Barcelona (UAB), Carrer de les Sitges s/n, Bellaterra, Spain.
The groundwater at the Sardas landfill in Huesca, Spain, is contaminated with benzene, chlorobenzenes, and hexachlorocyclohexane (HCH) isomers due to illegal waste dumping from a former lindane factory. In this study, microcosms using field-derived groundwater to evaluate in situ bioremediation were constructed. Anaerobic biostimulation with lactate successfully transformed α-, β-, δ-, and γ-HCH within two weeks, but failed to degrade benzene and less chlorinated benzenes, even with nutrient addition.
View Article and Find Full Text PDFDeciphering Co(III)-Catalyzed Oxidative C-H/C-H Annulation Towards Maleimide-Fused Imidazopyridine AEEgens.
Chemistry
December 2024
Department of chemistry, Indian Institute of Technology Guwahati, Guwahati, 781039, India.
A cobalt(III)-catalyzed dual C(sp)-H/C(sp)-H activation of 2-arylimidazopyridines and its annulation with N-substituted maleimides leads to polycyclic aromatic heterocycles. This sustainable oxidative annulation uses earth-abundant, less toxic, and cost-effective cobalt(III) catalyst that complement expensive 2nd and 3rd-row metals. This oxidative annulation features a broad substrate scope with very good functional group tolerance.
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