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The neurorepellent SLIT2 inhibits LPS-induced proinflammatory signaling in macrophages.
J Immunol
January 2025
Program in Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Macrophages are important mediators of immune responses with critical roles in the recognition and clearance of pathogens, as well as in the resolution of inflammation and wound healing. The neuronal guidance cue SLIT2 has been widely studied for its effects on immune cell functions, most notably directional cell migration. Recently, SLIT2 has been shown to directly enhance bacterial killing by macrophages, but the effects of SLIT2 on inflammatory activation of macrophages are less known.
View Article and Find Full Text PDFGenetically supported targets and drug repurposing for brain aging: A systematic study in the UK Biobank.
Sci Adv
March 2025
College of Computer Science and Technology, Zhejiang University, Hangzhou, China.
Brain age gap (BAG), the deviation between estimated brain age and chronological age, is a promising marker of brain health. However, the genetic architecture and reliable targets for brain aging remains poorly understood. In this study, we estimate magnetic resonance imaging (MRI)-based brain age using deep learning models trained on the UK Biobank and validated with three external datasets.
View Article and Find Full Text PDFQuantitative detection of the HVEM-BTLA checkpoint receptor cis-complex in human lymphocytes.
J Immunol
March 2025
Infectious and Inflammatory Diseases Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, United States.
The herpesvirus entry mediator (HVEM) (TNFRSF14) engagement of the checkpoint inhibitory receptor B and T lymphocyte attenuator (BTLA) limits immune responses of T and B lymphocytes. HVEM and BTLA form signaling complexes in trans and when coexpressed, complexes in cis, creating a unique immune checkpoint. The function of the HVEM-BTLA cis-complex is not well understood primarily due to a lack of reagents that specifically measure the HVEM-BTLA cis-complex.
View Article and Find Full Text PDFSingle-cell eQTL mapping in yeast reveals a tradeoff between growth and reproduction.
Elife
March 2025
Department of Human Genetics, University of California, Los Angeles, Los Angeles, United States.
Expression quantitative trait loci (eQTLs) provide a key bridge between noncoding DNA sequence variants and organismal traits. The effects of eQTLs can differ among tissues, cell types, and cellular states, but these differences are obscured by gene expression measurements in bulk populations. We developed a one-pot approach to map eQTLs in by single-cell RNA sequencing (scRNA-seq) and applied it to over 100,000 single cells from three crosses.
View Article and Find Full Text PDFProtocol for simultaneous detection of PRRS virus infection and dying cell populations by a flow cytometry double-staining approach.
STAR Protoc
March 2025
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USA. Electronic address:
When investigating cellular responses to viral infections, there is a need to quantitatively distinguish between dying cells, infected cells, and cells that are both infected and dying. Here, we present a protocol to simultaneously detect porcine reproductive and respiratory syndrome virus (PRRSV) infection and cell death using a flow cytometry double-staining approach. We describe steps for cell seeding, infection, and collection; staining dead cells; sample fixation; and cell permeabilization.
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