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Advancing carotenoid Quantification: A new method for semi-quantitative assessment of β -Carotene and lycopene content in food extracts.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2023
CONAHCYT, Laboratorio Regional para Estudio y Conservación de Germoplasma (GermoLab) del Centro de Investigación Científica de Yucatán, Parque Científico Tecnológico de Yucatán, Mexico. Electronic address:
Carotenoids, such as lycopene and β-carotene, have been widely recognized for their antioxidant properties and potential health benefits. Accurate quantification of carotenoids in plant extracts is essential for nutritional assessment, quality control, and research investigations. This study introduces an innovative method for quantifying lycopene and β-carotene, in plant extracts and aims to bridge the gap between complex and expensive carotenoid quantification techniques and the need for accessible methods that can be widely adopted.
View Article and Find Full Text PDFDetection and Semi-quantification of Lipids on High-Performance Thin-Layer Chromatography Plate using Ceric Ammonium Molybdate Staining.
Eur J Lipid Sci Technol
January 2023
Center for Translational Biomedical Research, University of North Carolina at Greensboro, North Carolina Research Campus, Kannapolis, NC 28081, USA.
It is desirable to quickly check the composition of lipids in small size samples, but achieving this is challenging using the existing staining methods. Herein, we developed a highly sensitive and semi-quantitative method for analysis of lipid samples with ceric ammonium molybdate (CAM) staining. The CAM detection method was systematically evaluated with a wide range of lipid classes including phospholipids, sphingolipids, glycerolipids, fatty acids (FA) and sterols, demonstrating high sensitivity, stability, and overall efficiency.
View Article and Find Full Text PDFFermentative Production of Diacylglycerol by Endophytic Fungi Screened from .
Foods
January 2023
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Diacylglycerol (DAG) production by microbial fermentation has broad development prospects. In the present study, five endophytic fungi which could accumulate DAG were screened from by using potato dextrose agar plate and flask cultivation in potato dextrose broth culture medium. The strains were biologically identified based on morphological features and semi-quantitative PCR.
View Article and Find Full Text PDFUrinary Glycosaminoglycans: Characterization and Quantification.
Methods Mol Biol
January 2023
Metabolic Platform, Biochemistry Laboratory, Faculty of Medicine, Cadi Ayyad University, Moroccan Association for Inherited Metabolic Diseases, Marrakech, Morocco.
The growing body of evidence supports the potential of using urinary glycosaminoglycans (uGAGs) levels as biomarkers to guide diagnosis and as predictive biomarkers of treatment efficacy. Recently, studies have shown that, in addition to MPS, the prognosis and treatment of cancers and viral infections, including COVID-19, are enabled by characterization and/or traits by GAGs. Reliable and accessible detection and assay protocols of urinary GAGs are therefore of great support for laboratory workers and clinicians.
View Article and Find Full Text PDFQuantification of rosmarinic acid from different plant species of lower Himalayan region and expression analysis of underlying L-Phenylalanine pathway.
Physiol Plant
September 2022
Department of Biotechnology, COMSATS University Islamabad, Abbottabad, Pakistan.
This study adopts a very effective high-performance liquid chromatography (HPLC) technique for the quantitative determination of rosmarinic acid (RA) and PCR-based amplification of biosynthetic key regulators in Isodon rugosus, Daphne mucronata, and Viburnum grandiflorum from the lower Himalayan regions. Rosmarinic acid is engaged in a variety of biological processes and has significant industrial significance. In this study, it was identified from crude methanolic extract using thin-layer chromatography with a standard, and its content was quantified using HPLC without interrupting spikes using a mixture of methanol and deionized water containing acetonitrile (70:30 v/v) and acetic acid (0.
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