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Biochem Biophys Res Commun
September 1994
Microbiology Laboratory, Indiana University School of Medicine-Evansville Center 47712.
Earlier studies from this laboratory demonstrated that a lumigenic detection method could utilize liquid scintillation counters for quantitation of DNA. Advantages included: 1) elimination of the need for radioactive probes, 2) rapid localization of spots by exposure of X-ray film and 3) longer shelf life of probe DNA. A major disadvantage of cutting spots from the membrane for luminometric assay is the destruction (or consumption) of the membrane; another disadvantage is the length of time spent handling the membrane while trying to cut out individual spots.
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