Growth of the yeast-like fungus Endomycopsis fibuliger 55-13 during continous cultivation was studied. The microorganism produced amylolytic enzymes during flow cultivation. As a result of the three-stage cultivation the productivity increased two-fold and the time of cultivation decreased by 6 hrs as compared with periodic cultivation.
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Plant Foods Hum Nutr
January 2025
Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana- Iztapalapa, Apartado Postal 55-534, Iztapalapa, CDMX, 09340, Mexico.
This study aimed to explore the effects of egg albumin protein addition (5, 15 and 20 g/100 g db) on the textural characteristics, as well as in the in vitro digestibility of protein and starch of wheat bread. Egg albumin addition resulted in smoother bread loaves as compared to traditional wheat bread. Reduced hardness and increased cohesiveness were correlated to the protein secondary structure, mainly with the content of β-sheets.
View Article and Find Full Text PDFBioresour Technol
January 2025
Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 81746-73441, Iran; Environmental Research Institute, University of Isfahan, Isfahan 81746-73441, Iran.
Tannin-containing sorghum grains, suitable for acetone-butanol-ethanol (ABE) production by Clostridium acetobutylicum, have required pretreatment to eliminate tannins inhibiting the strain's amylolytic activity. This study investigates biobutanol production enhancement by immobilizing enzymes on polydopamine-functionalized polyethersulfone (PES) membranes with magnetic nanoparticles for Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) processes. After multi-stage hot water treatment, TG3 sorghum (from the third stage) was used, where the enzyme-immobilized PES membrane produced 4.
View Article and Find Full Text PDFCurr Microbiol
December 2024
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, Rajasthan, India.
Amylases are pivotal enzymes with extensive industrial applications, including food processing, textile manufacturing, pharmaceuticals, and biofuel production. Traditional methods for enhancing amylase production in microbial strains often lack precision and efficiency. The advent of CRISPR/Cas9 technology has revolutionized genetic engineering, offering precise and targeted modifications to microbial genomes.
View Article and Find Full Text PDFBioresour Technol
December 2024
Waste to Bioproducts-Lab, Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), Università degli Studi di Padova, Agripolis, Viale dell'Università 16, 35020 Legnaro PD, Italy; Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address:
This study utilized a circular economy approach to convert unripe rice, a low-cost by-product of the rice milling industry, into biofuels using a biorefinery process. The recombinant yeast Saccharomyces cerevisiae ER T12.7 strain was tested for its ability to produce ethanol from unripe rice.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha 410114, Hunan Province, China; Hunan Provincial Engineering Technology Research Center of Aquatic Food Resources Processing, Changsha 410114, Hunan Province, China. Electronic address:
The effect of distribution behaviors of rice glutelin amyloid fibril aggregates (RAFA) on the structures and digestible process of wheat starch was investigated, and the interaction was revealed by molecular dynamics simulations. Rice glutelin (RG)/RAFA enhanced the long-range ordered structure of starch, and the relative crystallinity of gelatinized RAFA-wheat starch reached 14.35 %.
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