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Arsenic speciation in freshwater fish using high performance liquid chromatography and inductively coupled plasma mass spectrometry.
J Environ Sci (China)
July 2025
Department of Chemistry, Faculty of Science, University of Alberta, 11227 Saskatchewan Dr NW, Edmonton, Alberta, T6G 2G2, Canada; Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta, T6G 2G3, Canada. Electronic address:
Arsenic speciation in freshwater fish is crucial for providing meaningful consumption guidelines that allow the public to make informed decisions regarding its consumption. While marine fish have attracted much research interest due to their higher arsenic content, research on freshwater fish is limited due to the challenges in quantifying and identifying arsenic species present at trace levels. We describe here a sensitive method and its application to the quantification of arsenic species in freshwater fish.
View Article and Find Full Text PDFPavlovian-Type Learning in Environmental Bacteria: Regulation of Herbicide Resistance by Arsenic in Pseudomonas putida.
Environ Microbiol
December 2024
Systems Biology Department, Centro Nacional de Biotecnología-CSIC, Madrid, Spain.
The canonical arsRBC genes of the ars1 operon in Pseudomonas putida KT2440, which confer tolerance to arsenate and arsenite, are followed by a series of additional ORFs culminating in phoN1. The phoN1 gene encodes an acetyltransferase that imparts resistance to the glutamine synthetase inhibitor herbicide phosphinothricin (PPT). The co-expression of phoN1 and ars genes in response to environmental arsenic, along with the physiological effects, was analysed through transcriptomics of cells exposed to the oxyanion and phenotypic characterization of P.
View Article and Find Full Text PDFRole of the Sn-TiO/Ti-SnO Heterojunction in Enhancing the Photocatalytic Oxidation of Arsenite (As) through the Promotion of Charge Carrier Lifetime.
ACS Appl Mater Interfaces
December 2024
Department of Earth Science & Engineering, Imperial College London, London SW7 2AZ, U.K.
This study proposes the heterojunction photocatalyst, Sn-doped TiO/Ti-doped SnO (herein named SnTiO), as a promising alternative to pure TiO. SnTiO demonstrates improved light harvesting efficiency over TiO by generating longer-lived electron-hole (e-h) pairs, while also displaying a smaller band gap compared to pure TiO. Consequently, we show that it is a promising candidate for the photocatalytic oxidation (PCO) of As to the less toxic and more readily removable form As.
View Article and Find Full Text PDFYTHDF2 phase separation promotes arsenic-induced keratinocyte transformation in a poly-mA-dependent manner by inhibiting translational initiation of the key tumor suppressor PTEN.
J Hazard Mater
December 2024
Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address:
The phase separation of N-methyladenosine (mA) binding protein YTHDF2 plays a vital role in arsenic-induced skin damage, and YTHDF2 can bind to mA-methylated mRNA of tumor suppressor PTEN. However, whether and how YTHDF2 phase separation regulates PTEN involved in arsenic-induced malignant transformation of keratinocytes remains blank. Here, we established arsenite-induced transformation models with stable expression of wild-type YTHDF2 or mutant YTHDF2 protein in vitro and in vivo.
View Article and Find Full Text PDFStructural features within the NORAD long noncoding RNA underlie efficient repression of Pumilio activity.
Nat Struct Mol Biol
September 2024
Department of Immunology and Regenerative Biology and Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
Article Synopsis
- Long noncoding RNAs (lncRNAs) play crucial roles in mammalian cells, but their functional mechanisms and structural organization are not well understood.
- The study focuses on the NORAD lncRNA, which acts as a decoy for Pumilio proteins involved in regulating gene expression and genomic stability, particularly under stress conditions.
- Through analysis, researchers found that NORAD has a modular structure that allows it to interact with different RNAs and cluster binding sites, enhancing its regulatory function, which can inform the design of artificial lncRNAs for specific purposes.
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