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Microfluidic purification of genomic DNA.
Proc Natl Acad Sci U S A
January 2025
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611.
We describe a microfluidic device to extract DNA from a cell lysate, without the need for centrifuges, magnetic beads, or gels. Instead, separation is driven by transverse migration of DNA, which occurs when a polyelectrolyte solution flowing through a microfluidic channel is subjected to an electric field. The coupling of the weak shearing with the axial electric field is highly selective for long, flexible, charged molecules, of which DNA is the sole example in a typical cell lysate.
View Article and Find Full Text PDFEngineering Highly Aligned and Densely Populated Cardiac Muscle Bundles via Fibrin Remodeling in 3D-Printed Anisotropic Microfibrous Lattices.
Adv Mater
January 2025
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.
Replicating the structural and functional features of native myocardium, particularly its high-density cellular alignment and efficient electrical connectivity, is essential for engineering functional cardiac tissues. Here, novel electrohydrodynamically printed InterPore microfibrous lattices with anisotropic architectures are introduced to promote high-density cellular alignment and enhanced tissue interconnectivity. The interconnected pores in the microfibrous lattice enable dynamic, cell-mediated remodeling of fibrous hydrogels, resulting in continuous, mechanically stable tissue bundles.
View Article and Find Full Text PDFIntelligent Optimization Design Framework for Alternating Current Pulse Modulation Electrohydrodynamic Printing Process Parameters.
Small
January 2025
Key Laboratory for Micro/Nano Technology and System of Liaoning Province, Dalian University of Technology, Dalian, 116024, China.
To achieve efficient size tuning of printed microstructures on insulating substrates, an integrated process parameter intelligent optimization design framework for alternating current pulse modulation electrohydrodynamic (AC-EHD) printing is proposed for the first time. The framework is comprised of two stages: the construction of a prediction model and the acquisition of process parameters. The first stage employs the elk herd optimizer(EHO)-artificial neural network(ANN) to establish a mapping relationship between printing process parameters and the size of deposited droplets.
View Article and Find Full Text PDFMulti-material Electrohydrodynamic Printing of Bioelectronics with Sub-Microscale 3D Gold Pillars for In Vitro Extra- and Intra-Cellular Electrophysiological Recordings.
Adv Sci (Weinh)
January 2025
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.
Micro/nanoscale 3D bioelectrodes gain increasing interest for electrophysiological recording of electroactive cells. Although 3D printing has shown promise to flexibly fabricate 3D bioelectronics compared with conventional microfabrication, relatively-low resolution limits the printed bioelectrode for high-quality signal monitoring. Here, a novel multi-material electrohydrodynamic printing (EHDP) strategy is proposed to fabricate bioelectronics with sub-microscale 3D gold pillars for in vitro electrophysiological recordings.
View Article and Find Full Text PDFInvestigation on Influence Factors of Photo-Induced PLZT-Based Ion Drag Pump.
Micromachines (Basel)
November 2024
School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
The ion drag pump, as one kind of electrohydrodynamic pump, has received considerable attention in fluid applications due to its excellent pumping flow rate and pressure. However, there is a lack of systematic research about the factors that influence pumping performance of the ion drag pump. Here, a photo-induced ion drag pump based on the PLZT ceramic is proposed by combining the photoelectric effect and field emission phenomenon.
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