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Principles of ion binding to RNA inferred from the analysis of a 1.55 Å resolution bacterial ribosome structure - Part I: Mg2.
Nucleic Acids Res
December 2024
Université de Strasbourg, Architecture et Réactivité de l'ARN, Institut de Biologie Moléculaire et Cellulaire du CNRS, 2 Allée Konrad Roentgen, 67084 Strasbourg, France.
The importance of Mg2+ ions for RNA structure and function cannot be overstated. Several attempts were made to establish a comprehensive Mg2+ binding site classification. However, such descriptions were hampered by poorly modelled ion binding sites as observed in a recent cryo-EM 1.
View Article and Find Full Text PDFNoninvasive Assessment of Liver Fibrosis in Patients With Iron Overload.
Ultrasound Med Biol
March 2025
Center for Ultrasound Research and Translation, Massachusetts General Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Objective: We assessed the diagnostic performance of ultrasound two-dimensional shear wave elastography (US 2D-SWE) to predict clinically significant fibrosis (CSF) in patients with serologic iron overload (SIO) and the subgroup with histologic liver iron overload (LIO).
Methods: A single-center retrospective cross-sectional study of adults with SIO (serum ferritin ≥ 200 ng/mL in females and ≥ 300 ng/mL in males) and suspected chronic liver disease with nonfocal liver biopsy results and US 2D-SWE exams within 1 year was performed. Histopathological fibrosis stage ≥2 and liver iron ≥2+ was considered CSF and LIO, respectively.
International survey on diagnostic reference levels based on clinical indications in plain radiography.
Eur Radiol
December 2024
Institute of Diagnostic and Interventional Radiology, Municipal Hospital Magdeburg, Birkenallee 34, 39130, Magdeburg, Germany.
Purpose: To collect and analyze radiation dose-related data as part of international cooperation; to define diagnostic reference levels (DRL) for 24 X-ray projections in plain radiography (DX) considering anatomical region, clinical task, and procedural technique; and to harmonize the exposure practice across country borders.
Methods: A multicenter study was performed in Austria, Germany, Italy, and Switzerland in 2022-23 to provide dose-related data. Healthcare facilities were asked to provide processed data from their dose management systems.
Recent trends in representation of female radiologists in radiology annual scientific meetings.
Curr Probl Diagn Radiol
November 2024
Massachusetts General Hospital, Boston, MA, USA, 02114; Harvard Medical School, Boston, MA, USA, 02138.
Article Synopsis
- The study aimed to assess how well women were represented as speakers at U.S. radiology annual scientific meetings from 2019 to 2022.
- At the Society of Interventional Radiology (SIR), female speaker representation rose from 20.0% to 26.5%, while the American Roentgen Ray Society (ARRS) saw fluctuations, peaking at 48.5% in 2022 after dropping in previous years.
- The findings highlight a general improvement in female speaker representation, but emphasize the need for ongoing advocacy and strategies to maintain and promote gender equality in radiology.
Simultaneous determination of cytosolic aminoacyl-tRNA synthetase activities by LC-MS/MS.
Nucleic Acids Res
December 2024
Department Laboratory Medicine, Laboratory Genetic Metabolic Diseases, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands.
In recent years, pathogenic variants in ARS genes, encoding aminoacyl-tRNA synthetases (aaRSs), have been associated with human disease. Patients harbouring pathogenic variants in ARS genes have clinical signs partly unique to certain aaRSs defects, partly overlapping between the different aaRSs defects. Diagnosis relies mostly on genetics and remains challenging, often requiring functional validation of new ARS variants.
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