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Testing AuxInYeast Synthetic Biology Strains via Fluorescence Flow Cytometry.
Cold Spring Harb Protoc
March 2025
Department of Ecology, University of Chicago, Chicago, Illinois 60637, USA.
Understanding how the auxin hormone signaling pathway components come together to orchestrate cellular responses is key to engineering the growth and development of maize. Although a variety of techniques exist to measure auxin activities in plants, many are time- and resource-intensive or do not easily allow for high-throughput quantitative measurement of component libraries. The AuxInYeast system is a synthetic biology tool that facilitates complex biochemical analysis of the auxin hormone signaling pathway from essentially any plant.
View Article and Find Full Text PDFBuilding AuxInYeast Synthetic Biology Strains for Biochemical Characterization of Maize Auxin Hormone Signaling Components.
Cold Spring Harb Protoc
March 2025
Department of Biology, Whitman College, Walla Walla, Washington 99362, USA
The AuxInYeast system is a synthetic biology tool that facilitates complex biochemical analysis of the plant auxin hormone signaling pathway. As a plant synthetic biology chassis, yeast offers rapid growth, well-established genetic and biochemical tools, and core eukaryotic cellular machinery compatible with heterologous plant gene expression. The AuxInYeast system for maize consists of yeast cells containing the minimal necessary set of plant auxin signaling parts: a receptor (ZmTIR1/AFB), repressor (ZmIAA), corepressor (REL2), transcription factor (ZmARF), and auxin response -element (auxRE).
View Article and Find Full Text PDFFunctional analysis of the epsilon glutathione S-transferases in the adaptation of Spodoptera litura to xanthotoxin.
Insect Biochem Mol Biol
March 2025
Key Laboratory of Agri-products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China. Electronic address:
Through long-term coevolution with host plants, insects have evolved sophisticated detoxification systems to counteract plant secondary metabolites (PSMs). However, the precise mechanisms underlying these adaptations remain incompletely characterized. Our previous research identified epsilon glutathione S-transferases (GSTes) as critical mediators of xanthotoxin adaptation in Spodoptera litura, a model linear furanocoumarin.
View Article and Find Full Text PDFImpact of gold nanoparticle exposure on the development pancreas and kidney: Dose-dependent;oxidative stress; miRNA expression and Nrf2/ARE Signalling.
Int Immunopharmacol
March 2025
Department of Anatomy, College Medicine, King Khalid University, Abha 62529, Saudi Arabia. Electronic address:
Background: The widespread use of gold nanoparticles (AuNPs) in consumer and medical products necessitates investigation into their potential developmental toxicity.
Aim Of The Work: This study investigated the systemic effects of in-utero AuNP exposure on developing male rat offspring, focusing on metabolic, organ-specific, and cellular pathways.
Materials And Methods: Pregnant rats were intravenously administered AuNPs (5, 10, 15, or 20 mg/kg) or saline from gestational day 1 to birth.
"Unveiling the impacts of climatic cold events on the cardiovascular health in animal models".
Sci Total Environ
March 2025
Department of Environmental Studies, University of Delhi, New Delhi, India. Electronic address:
Climate change is increasingly driving extreme weather events, leading to drastic temperature fluctuations worldwide. While overall temperatures rise, many regions are simultaneously experiencing severe cold spells that threaten the health of human populations, especially to vulnerable populations including the elderly and those with pre-existing conditions. Exposure to cold stress triggers significant physiological and biochemical disruptions.
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