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[Effect of nerve growth factor and amizil on the viability and metabolism of cultured spinal ganglia]. | LitMetric

AI Article Synopsis

  • Spinal ganglia from 9-day chick embryos were cultured using floating rafts in two different media: one control and one with either amizyl or a neurogrowth factor.
  • Amizyl treatment resulted in more surviving neurons, an increase in monoaminoxidase presence, and elevated activities of NAD-diaphorase, along with some rise in lactic and isocitric dehydrogenases.
  • The neurogrowth factor increased nerve cells with acetylcholinesterase, boosted NAD-diaphorase and lactic dehydrogenase activities, but slightly suppressed succinic dehydrogenase activity.

Article Abstract

Spinal ganlia of a 9-day chick embryo were cultivated by the method of "floating rafts" in common medium (control) and in the medium containing amizyl (100 microgram/ml) or a neuregrowth factor (50 microgram/ml). With the action of amizyl there proved to be an increase in the number of surviving neurons; the majority of these neurons contained monoaminoxidase; there was a rise of NAD-diaphorase activity, and, to a lesser extent, of lactic dehydrogenase and isocitric dehydrogenase activities. The neurogrowth factor caused an increase in the number of nerve cells with acetylcholinesterase; there was an elevation of NAD-diaphorase and some rise of malic dehydrogenase activities; the activity of lactic dehydrogenase became maximal; as to succinic dehydrogenase--its activity was somewhat suppressed.

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