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Retention mechanism in slalom chromatography: Perspectives on the characterization of large DNA and RNA biopolymers in cell and gene therapy.
J Chromatogr A
January 2025
Waters Corporation, Instrument/Core Research/Fundamental, Milford, MA, 01757, USA. Electronic address:
Significant progress has been made in the last two decades in producing small (<2μm), high-purity, and low-adsorption particles, columns and system hardware, for ultra-high pressure liquid chromatography (UHPLC). Simultaneously, the recent rapid expansion of cell and gene therapies for treating diseases necessitates novel analytical technologies for analyzing large (>2 kbp) plasmid double-stranded (ds) DNA (which encodes for the in vitro transcription (IVT) of single-stranded (ss) mRNA therapeutics) and dsRNAs (related to IVT production impurities) biopolymers. In this context, slalom chromatography (SC), a retention mode co-discovered in 1988, is being revitalized using the most advanced column technologies for improved determination of the critical quality attributes (CQAs) of such new therapeutics.
View Article and Find Full Text PDFExploring Lysine Incorporation as a Strategy to Mitigate Postsynthetic Halide Exchange in Lead-Halide Hybrid Perovskites.
ACS Appl Mater Interfaces
January 2025
Department of Materials Science and Engineering and the Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Lead-halide hybrid perovskites (RNHPbX, X = halide, e.g., Cl, Br, I; R = organic moiety) show promise for next-generation optoelectronic devices due to their simple synthesis routes, strong light absorption, and high photoluminescence quantum yield.
View Article and Find Full Text PDFAmino Acid Patterns in Children with Autistic Spectrum Disorder: A Preliminary Biochemical Evaluation.
Nutrients
January 2025
Department of Pediatrics, Buzzi Children's Hospital, 20154 Milan, Italy.
Background: The metabolism of plasma amino acid (AA) in children with autism spectrum disorder (ASD) has been extensively investigated, yielding inconclusive results. This study aims to characterize the metabolic alterations in AA profiles among early-diagnosed children with ASD and compare the findings with those from non-ASD children.
Methods: We analyzed plasma AA profiles, measured by ion exchange chromatography, from 1242 ASD children (median age = 4 years; 81% male).
[A novel carbonyl reductase for the synthesis of ()-tolvaptan].
Sheng Wu Gong Cheng Xue Bao
January 2025
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry and Materials Sciences, Hebei University, Baoding 071002, Hebei, China.
Screening carbonyl reductases with the ability to catalyze the reduction of complex carbonyl compounds is of great significance for the biosynthesis of -tolvaptan(-TVP). In this study, the target carbonyl reductase in the crude enzyme extract of rabbit liver was separated, purified, and identified by ammonium sulfate precipitation, gel-filtration chromatography, ion exchange chromatography, affinity chromatography, and protein mass spectrometry. With the rabbit liver genome as the template, the gene encoding the carbonyl reductase was amplified by PCR and the recombinant strain was successfully constructed.
View Article and Find Full Text PDFPhytochemicals that Regulate Oviposition Mistakes of Eurema mandarina on Oxalis corniculata.
J Chem Ecol
January 2025
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashihiroshima, 739-8528, Japan.
Eurema mandarina is a pierid butterfly that primarily feeds on plants in the family Fabaceae. In mainland Japan, adult females preferentially lay eggs on Albizia julibrissin and Lespedeza cuneata. In the field, females may oviposit on non-fabaceous plants, although rarely.
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