The feasibility of a technique for autoradiography of diffusible substances has been further tested by analysing the localization of steroids in rat testes with the light-and electron-microscope. Testes of rats were perfused with tritiated testosterone (3 min) followed by 15-min perfusion with buffer containing a 100-fold of unlabelled testosterone. Tissue samples were frozen, freeze dried, fixed in osmium vapour and embedded in Epon. To exclude extraction of steroids, contact with water and other solvents was prevented during cutting of thin sections on an ultracryotome and further treatments for autoradiography. Light- and electron-microscopic observations indicate that the highest concentration of labelled testosterone was present within the basal parts of the Sertoli cell cytoplasm and in lipid inclusions of Sertoli cells within the seminiferous tubules. This is the first account of autoradiography of steroids at the electron-microscope level.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1242/jcs.26.1.339 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-throughput analysis of the steroid profile in placental cell cultures to evaluate endocrine disrupting effects of contaminant exposure.
J Chromatogr A
March 2022
Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain. Electronic address:
Human placental JEG-3 cells conserve a high P450 aromatase activity and are therefore suitable to evaluate how contaminants may interfere with the routes involved in estrogen synthesis during pregnancy. This has been traditionally assessed by measuring aromatase activity through the amount of tritiated water (HO) formed during the aromatization of 1β-H-androst-4-ene-3,17-dione (H-AD). This work presents a greener and safer analytical approach for this purpose, which consists of the determination of the trace amounts of the steroids (estradiol, estrone, testosterone, and androstenedione) present in the culture medium.
View Article and Find Full Text PDFThe Uptake of Ethinyl-Estradiol and Cortisol From Water by Mussels ( spp.).
Front Endocrinol (Lausanne)
February 2022
Centre for Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Weymouth, United Kingdom.
Previous toxicokinetic studies have shown that mussels ( spp.) can readily absorb the three main mammalian sex steroids, estradiol (E), testosterone (T) and progesterone (P) from water. They also have a strong ability to store E and the 5α-reduced metabolites of T and P in the form of fatty acid esters.
View Article and Find Full Text PDFCharacterizing the timing of yolk testosterone metabolism and the effects of etiocholanolone on development in avian eggs.
J Exp Biol
February 2020
School of Biological Sciences, Illinois State University, Normal, IL 61790, USA
Maternal transfer of steroids to eggs can elicit permanent effects on offspring phenotype. Although testosterone was thought to be a key mediator of maternal effects in birds, we now know that vertebrate embryos actively regulate their exposure to maternal testosterone through steroid metabolism, suggesting testosterone metabolites, not testosterone, may elicit the observed phenotypic effects. To address the role steroid metabolism plays in mediating yolk testosterone effects, we used European starling () eggs to characterize the timing of testosterone metabolism and determine whether etiocholanolone, a prominent metabolite of testosterone in avian embryos, is capable of affecting early embryonic development.
View Article and Find Full Text PDFThe effect of finasteride and dutasteride on the synthesis of neurosteroids by glioblastoma cells.
Steroids
March 2020
Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, Avenida IPN # 2508, Ciudad de Mexico 07360, Mexico. Electronic address:
Glioblastoma (GBM) is the most aggressive local brain tumor and effective treatments are lacking. Many studies have proposed an important participation of steroid hormones in the development of gliomas. Evidence was provided by statistics analysis where the incidence in adult population is 50% higher in men than in women.
View Article and Find Full Text PDFHigher environmental temperatures promote acceleration of spermatogenesis in vivo in mice (Mus musculus).
J Therm Biol
October 2018
Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil; National Institute for Amazonian Research (INPA), Manaus, AM, Brazil. Electronic address:
Temperature is considered a crucial modulator of reproductive activity and testis homeostasis. It is well known that elevated temperatures cause several effects on testicular components, particularly on germ cells, which might lead to the impairment of spermatogenesis and loss of male fertility. The present study aimed to evaluate the effects of different environmental temperatures on several morphofunctional testis parameters, with emphasis on duration of spermatogenesis and spermatogenic efficiency.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!