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Validation of an in house iELISA for serodiagnosis of caprine brucellosis and evaluation of the performance of a B. neotomae lysate for the detection of anti-smooth Brucella specific antibodies in ruminants.
Vet Microbiol
January 2025
Instituto de Patobiología - Instituto de Patobiología Veterinaria (IP-IPVET), UEDD INTA-Conicet, Nicolás Repetto y de Los Reseros s/n (B1686), Hurlingham, Buenos Aires, Argentina; CONICET, Godoy Cruz 2290 (C1425FQB), CABA, Argentina. Electronic address:
Enzyme-linked immunosorbent assay (ELISA) is a widely used and effective tool for detection of anti-Brucella antibodies in serum, easy to perform with high sensitivity and specificity. In this study, we validated an in-house indirect ELISA using B. melitensis whole cell lysate as antigen (Bm-WCL iELISA) for the serodiagnosis of caprine brucellosis and evaluated the use of BSL-2 B.
View Article and Find Full Text PDFArticle Synopsis
- Glioblastoma (GB) is a highly aggressive brain tumor with poor treatment options and low survival rates, leading to a need for innovative drug delivery methods.
- This study focuses on creating triphenylphosphonium (TPP)-conjugated chitosan nanoparticles to effectively deliver temozolomide (TMZ) specifically to GB cells, using a Quality-by-Design approach for development.
- The optimized nanoparticles showed favorable characteristics, including a small size and high drug entrapment efficiency, while demonstrating improved retention in the nasal mucosa and enhanced cytotoxic effects compared to TMZ alone.
Efficacy of Carbonate Buffer Mixture in Preventing Hoof Lamella Injury Associated with Subacute Ruminal Acidosis in Dairy Goats.
Vet Sci
August 2024
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Subacute ruminal acidosis (SARA) is a prevalent metabolic disorder in highly productive dairy cows that results in serious issues, including hoof lamellar injuries. This study aimed to investigate the efficacy of a carbonate buffer mixture (CBM) in preventing hoof lamella injury in dairy goats, a species also susceptible to SARA due to similar feeding practices over a 17-week period. Twenty-four healthy dairy goats were randomly assigned to three groups: control, SARA, and CBM groups.
View Article and Find Full Text PDFBackground: This study delves into the epidemiology and pathomorphologic characteristics of peste des petits ruminants (PPR) in western Algeria, a viral disease that constantly threatens small animals in Africa, the Middle East, and Asia.
Aim: The purpose of this investigation was to evaluate the epidemiology of PPR in western Algeria and to understand the pathomorphological lesions in naturally infected small ruminants.
Methods: An online survey conducted via google forms and shared with veterinarians in the wilaya of Tiaret, provided insights into the prevalence and clinical manifestations of PPR.
Effect of D,L methionine and ammonium chloride on urine acidification, urinary fractional excretion of calcium, and blood bicarbonate in clinically healthy goats.
Am J Vet Res
November 2024
Department of Pathobiology and Population Medicine, Mississippi State University, Starkville, MS.
Background: Urinary acidification with ammonium chloride (AC) for urolith dissolution is a common treatment for goats with urolithiasis. Studies have reported increased fractional excretion of calcium (FECa) following AC administration, which could increase calcium-based urolithiasis. D,L methionine (MET) may result in similar acidification with less calcium excretion.
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