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Increased plugging latency in cycling epiERα-/- (Esr1f/-Wnt7aCre/+) mice.
Reproduction
January 2025
X Ye, Department of Physiology and Pharmacology, University of Georgia, Athens, United States.
Wnt7a-Cre is a commonly used for generating uterine epithelial conditional knockout mice, such as epiERα-/- (Esr1f/-Wnt7aCre/+) and epiPR-/- (Pgrf/-Wnt7aCre/+). We noticed that epiERα-/- females, but not epiPR-/- females, have prolonged plugging latency, which is the duration between continuous cohabitation and detection of the first vaginal plug (a sign of mating). Mating occurs in proestrus and/or estrus stages of the estrous cycle.
View Article and Find Full Text PDFComparison of Liquid-Based Cytology EASYPREP and SurePath for Detection of High-Risk HPV Genotypes.
Ann Clin Lab Sci
November 2024
Department of Laboratory Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea
Objective: Molecular testing for high-risk human papillomavirus (hrHPV) genotypes is important for cervical cancer screening. In this study, we compared the HPV detection rates using real-time PCR in cervical samples collected using two different liquid-based cytology (LBC) kits.
Methods: Cervical swab specimens were prospectively collected using the SurePath and EASYPREP collection kits.
Reproduction control in a feral feline population using an anti-GnRH vaccine.
Theriogenology
January 2025
Instituto de Investigaciones en Reproducción Animal, FCV, UNLP, Calle 60 y 118, La Plata, B1900AVW, Buenos Aires, Argentina; Universidad del Chaco Austral, Argentina. Electronic address:
This study aimed to evaluate the use of the Improvac® vaccine to avoid heat and pregnancies in queens and fertility in males during the breeding season. Twenty-eight intact animals were divided into treated males (G1, n = 7), treated females (G2, n = 18), and untreated males (G3, n = 3) that were untamed and could not be captured for immunization. In cats from the G1 group, the testicular volume (337.
View Article and Find Full Text PDFReproducibility and Consistency of Isolation Protocols for Fibroblasts, Smooth Muscle Cells, and Epithelial Cells from the Human Vagina.
Cells
January 2025
Reproductive Biology Laboratory, Amsterdam UMC-Location AMC, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
(1) Background: For the reconstruction of a human vagina, various surgical procedures are available that are often associated with complications due to their failure to mimic the physiology of the human vagina. We recently developed a vascularized, organ-specific matrix from healthy human vaginal wall tissue with suitable biomechanical properties. A superior graft would require further extensive colonization with autologous vaginal cells to reduce complications upon implantation.
View Article and Find Full Text PDFThe Relationship Between Cervicovaginal Infection, Human Papillomavirus Infection and Cervical Intraepithelial Neoplasia in Romanian Women.
Diseases
January 2025
Department of Speciality Disciplines, "Titu Maiorescu" University, 031593 Bucharest, Romania.
Cervical intraepithelial neoplasia (CIN) is a premalignant cervical condition closely linked to persistent high-risk HPV infection, a major risk factor for cervical cancer. This study aims to investigate the relationship between cervicovaginal infections, HPV infection, and CIN development in 94 Romanian women with cervical lesions. Comprehensive assessments included HPV genotyping, cytology, colposcopy, and histopathology.
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