Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Deep mutational scanning of the Trypanosoma brucei developmental regulator RBP6 reveals an essential disordered region influenced by positive residues.
Nat Commun
January 2025
Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, 06536, USA.
To regain infectivity, Trypanosoma brucei, the pathogen causing Human and Animal African trypanosomiasis, undergoes a complex developmental program within the tsetse fly known as metacyclogenesis. RNA-binding protein 6 (RBP6) is a potent orchestrator of this process, however, an understanding of its functionally important domains and their mutational constraints is lacking. Here, we perform deep mutational scanning of the entire RBP6 primary structure.
View Article and Find Full Text PDFAnalysis and Identification of Polyphenols in Fragaria, Rubus, and Ribes Species by Nontargeted UHPLC-PDA-MS Metabolite Profiling.
Methods Mol Biol
January 2025
Environmental and Biochemical Sciences, James Hutton Institute, Dundee, Scotland, UK.
Profiling of secondary metabolites within Fragaria sp. (strawberry), Rubus sp. (raspberries and blackberries), and Ribes sp.
View Article and Find Full Text PDFBranched-chain and Aromatic Amino Acids in Blood and Seminal Plasma are Associated with Sperm Parameters; A Practice within a Fertility Clinic Considering the Metabolic Syndrome.
Reprod Sci
January 2025
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
The metabolomic approach has recently been used in the assessment of semen quality and male fertility. Additionally, the crucial roles of branched-chain amino acids (BCAAs) and aromatic amino acids (AAAs) in metabolic syndrome (MetS) were reported. However, little information exists about the association between BCAAs and AAAs with semen parameters, particularly in men with and without MetS.
View Article and Find Full Text PDFFish gut symbiotic bacterium Bacillus thuringiensis: RSM optimization for its extracellular lipase enzyme production, lipase-protein purification, characterization, and docking analysis.
Int J Biol Macromol
January 2025
State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu 610059, Sichuan, China.
Lipase enzymes play a vital role in digestion and nutrient metabolism in host organisms, with symbiotic bacteria producing abundant enzymes, carbohydrates, vitamins, and other nutrients. This study aimed to isolate, identify, and screen lipase-producing bacteria from the gut of Systomus sarana, optimize enzyme production using Response Surface Methodology (RSM), and characterize the extracted lipase protein. A total of 11 bacterial strains were isolated and identified through 16S rRNA sequencing.
View Article and Find Full Text PDFEffects of melatonin on the pharmacokinetics and amino acid metabolism profile of vigabatrin in rats.
Toxicol Appl Pharmacol
January 2025
School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, China; Anhui Provincial Laboratory of Inflammatory and Immune Disease, Anhui Institute of Innovative Drugs, Hefei, Anhui 230032, China; The Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Medical University, Hefei, Anhui 230032, PR China. Electronic address:
Objectives: Investigating the effect of melatonin (MLT) on the pharmacokinetics and related neurotransmitter and amino acid metabolism of vigabatrin (VGB) in epileptic rats in vivo.
Methods: High performance liquid chromatography was used to examine the pharmacokinetics and tissue distribution of VGB after intragastric administration dosing (50，100，200) mg/kg singly or in combination with melatonin (20 mg/kg) in rats. The single-compartment model of first-order elimination was fitted with the nonlinear mixed-effect model of first-order estimation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!