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Detection and characterization of pathogenic Bacillus haynesii from Tribulus terrestris extract: ways to reduce its levels.
Braz J Microbiol
January 2025
Innovation and Drug Discovery, Sava Healthcare Limited, Research Center, MIDC, Block D1, Plot No. 17/6, Chinchwad, Pune, 411019, India.
Plant parts such as roots, bark, leaves, flowers, and fruits that hold ethnopharmacological significance are naturally prone to microbial contamination, influenced by environmental factors like moisture and humidity. This study focuses on assessing the microbial load in the raw material of Tribulus terrestris (TT). The primary bacterium isolated from the pulverized raw material was identified as Bacillus haynesii through 16S rRNA sequencing.
View Article and Find Full Text PDFIsolation and structure determination of a new antibacterial lanthipeptide derived from the marine derived bacterium Lysinibacillus sp.CTST325.
World J Microbiol Biotechnol
January 2025
Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
Marine resources are attractive for screening new useful bacteria. From a marine sediment sample, we performed isolation and screening of bacterial strains in search of new bioactive compounds. HPLC and ESI-MS analysis indicated that the new bacterium, Lysinibacillus sp.
View Article and Find Full Text PDFTwo new enzymes that liberate undecaprenyl-phosphate to replenish the carrier lipid pool during envelope stress.
mBio
January 2025
Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
The 55-carbon isoprenoid, undecaprenyl-phosphate (UndP), is a universal carrier lipid that ferries most glycans and glycopolymers across the cytoplasmic membrane in bacteria. In addition to peptidoglycan precursors, UndP transports O-antigen, capsule, wall teichoic acids, and sugar modifications. How this shared but limited lipid is distributed among competing pathways is just beginning to be elucidated.
View Article and Find Full Text PDFCharacterization of extended-spectrum beta-lactamase-producing from recreational water in Athens, GA, using an undergraduate laboratory module.
J Microbiol Biol Educ
January 2025
Department of Microbiology, University of Georgia, Athens, Georgia, USA.
We present a laboratory module that uses isolation of antibiotic-resistant bacteria from locally collected stream water samples to introduce undergraduate students to basic microbiological culture-based and molecular techniques. This module also educates them on the global public health threat of antibiotic-resistant organisms. Through eight laboratory sessions, students are involved in quality testing of water sources from their neighborhoods, followed by isolation of extended-spectrum beta-lactamase-producing .
View Article and Find Full Text PDF<b>Background and Objective:</b> Peatlands are unique ecosystems rich in microbial diversity, including bacteria with potential antibiotic activity. This study focuses on the isolation and characterization of bacteria from Indonesian peat soil, particularly their potential to produce antibiotics against multidrug-resistant (MDR) pathogens, including Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA). <b>Materials and Methods:</b> Bacterial isolates were rejuvenated on nutrient agar and subjected to antimicrobial activity testing using the Bauer & Kirby diffusion method against MRSA.
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