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Systematic characterization of indel variants using a yeast-based protein folding sensor.
Structure
December 2024
Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark. Electronic address:
Gene variants resulting in insertions or deletions of amino acid residues (indels) have important consequences for evolution and are often linked to disease, yet, compared to missense variants, the effects of indels are poorly understood and predicted. We developed a sensitive protein folding sensor based on the complementation of uracil auxotrophy in yeast by circular permutated orotate phosphoribosyltransferase (CPOP). The sensor reports on the folding of disease-linked missense variants and de-novo-designed proteins.
View Article and Find Full Text PDFGreen microalga conserves substrate uptake pattern but changes their metabolic uses across trophic transition.
Front Microbiol
November 2024
Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
The terrestrial green alga is an emerging model species with potential applications including production of triacylglycerol or astaxanthin. How interacts with the diverse substrates during trophic transitions is unknown. To characterize its substrate utilization and secretion dynamics, we cultivated the alga in a soil-based defined medium in transition between conditions with and without glucose supplementation.
View Article and Find Full Text PDFGut Microbiota and Related Metabolites in Children With Egg White Sensitization.
Pediatr Infect Dis J
December 2024
Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Background: We hypothesized that food sensitization in children could be linked to specific gut microbiota. The objective of this study is to assess a group of children with egg white sensitization (ES) from the microbiological and biochemical-metabolic standpoint, applying the microbiota and metabolomics approach to studying the intestinal contents of the feces.
Methods: Twenty-eight toddlers with ES (mean age 13.
Predictive metabolite signatures for risk of progression to active TB from QuantiFERON supernatants of household contacts of TB patients.
Emerg Microbes Infect
December 2025
Department of Virology and Biotechnology, National Institute for Research in Tuberculosis, Indian Council of Medical Research (ICMR), Chennai, India.
The identification of individuals with the greatest risk of progression to active tuberculosis (TB) disease from the huge reservoir of () infected individuals continues to remain an arduous ascent in the global effort to control TB. In a two-year prospective study, we analysed metabolic profiles in the unstimulated and TB antigen stimulated QuantiFERON supernatants of 14 healthy household contacts (HHCs) who progressed to TB disease (Progressors) and 14 HHCs who remained healthy (Non-Progressors). We identified 21 significantly dysregulated metabolites in the TB antigen-stimulated QuantiFERON supernatants of Progressors, of which the combination of Malic acid and N-Arachidonoylglycine had maximum AUC of 0.
View Article and Find Full Text PDFSystematic Metabolic Engineering for Enhanced Cytidine 5'-Monophosphate Production in .
J Agric Food Chem
December 2024
School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
Article Synopsis
- Cytidine 5'-monophosphate (5'-CMP) is an important compound for food and pharmaceutical applications, and its production has been improved in engineered strains of bacteria.
- In the study, the engineered strain CM006 showed a 134.7-fold increase in 5'-CMP production by blocking its degradation pathway, and further modifications led to higher yields of the compound.
- The highest reported titer of 5'-CMP reached 417.9 mg/L in strain CM012, demonstrating effective methods for sustainable industrial production of this valuable nucleotide.
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