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Macrophage activity modulation via synergistic effect of a porous substrate and low-field laser therapy.
Acta Bioeng Biomech
June 2024
1Institute of Applied Sciences, Academy of Physical Education, Kraków, Poland.
: The aim of this study was to investigate the effect of substrate - polycaprolactone (PCL)-based porous membrane modified with rosmarinic acid (RA), (PCL-RA) and to determine the optimal values of low field laser irradiation (LLLT) as stimulators of biological response of RAW 264.7 macrophages. : The porous polymer membrane was obtained by the phase inversion method, the addition of rosmarinic acid was 1%wt.
View Article and Find Full Text PDFThe importance of method selection when estimating diet composition with quantitative fatty acid signature analysis.
PLoS One
January 2025
Alaska Science Center, U.S. Geological Survey, Anchorage, AK, United States of America.
Quantitative fatty acid signature analysis (QFASA) is a common method of estimating the composition of prey species in the diets of consumers from polar and temperate ecosystems in which lipids are an important source of energy. A key characteristic of QFASA is that the large number of fatty acids that typically comprise lipids permits the dietary contributions of a correspondingly large number of prey types to be estimated. Several modifications to the original QFASA methods have been suggested in the literature and a significant extension of the original model published in 2017 allows simultaneous estimation of both diet proportions and calibration coefficients, which are metabolic constants in the model whose values must otherwise be estimated in independent feeding experiments.
View Article and Find Full Text PDFNanopore-Based High Resolution Detection of Multiple Post-Translational Modifications in Proteins.
Angew Chem Int Ed Engl
January 2025
Xihua University, College of Food and Bioengineering, CHINA.
Protein post-translational modifications (PTMs) play crucial roles in various cellular processes. Despite their significance, only a few PTMs have been extensively studied at the proteome level, primarily due to the scarcity of reliable, convenient, and low-cost sensing methods. Here, we present a straightforward and effective strategy for detecting PTMs on short peptides through host-guest interaction-assisted nanopore sensing.
View Article and Find Full Text PDFCombining Multifrequency Magnetic Resonance Elastography With Automatic Segmentation to Assess Renal Function in Patients With Chronic Kidney Disease.
J Magn Reson Imaging
January 2025
Department of Radiology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine (Shenzhen Traditional Chinese Medicine Hospital), Shenzhen, China.
Background: Multifrequency MR elastography (mMRE) enables noninvasive quantification of renal stiffness in patients with chronic kidney disease (CKD). Manual segmentation of the kidneys on mMRE is time-consuming and prone to increased interobserver variability.
Purpose: To evaluate the performance of mMRE combined with automatic segmentation in assessing CKD severity.
Aerolysin Nanopore Electrochemistry.
Acc Chem Res
January 2025
Molecular Sensing and Imaging Center, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
ConspectusIons are the crucial signaling components for living organisms. In cells, their transportation across pore-forming membrane proteins is vital for regulating physiological functions, such as generating ionic current signals in response to target molecule recognition. This ion transport is affected by confined interactions and local environments within the protein pore.
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