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Imaging flow cytometry reveals the mechanism of equine arteritis virus entry and internalization.
Sci Rep
January 2025
Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375, Wroclaw, Poland.
The process of viral entry into host cells is crucial for the establishment of infection and the determination of viral pathogenicity. A comprehensive understanding of entry pathways is fundamental for the development of novel therapeutic strategies. Standard techniques for investigating viral entry include confocal microscopy and flow cytometry, both of which provide complementary qualitative and quantitative data.
View Article and Find Full Text PDFDifferential Cytotoxicity and Inflammatory Responses to Particulate Matter Components in Airway Structural Cells.
Int J Mol Sci
January 2025
Lung Biology, Department of Experimental Medical Sciences, Lund University, 221 84 Lund, Sweden.
Particulate matter (PM) is a major component of ambient air pollution. PM exposure is linked to numerous adverse health effects, including chronic lung diseases. Air quality guidelines designed to regulate levels of ambient PM are currently based on the mass concentration of different particle sizes, independent of their origin and chemical composition.
View Article and Find Full Text PDFEffects of green manuring on chemical characteristics and microecology of tobacco-growing soil in central henan.
BMC Microbiol
January 2025
College of Tobacco Science/Research Center for Tobacco Harm Reduction/Tobacco Cultivation Key Laboratory of China Tobacco, Henan Agricultural University, Zhengzhou, Henan, 450002, People's Republic of China.
Objective: This study explored green manuring effects on microecology, carbon/nitrogen levels, and enzyme activity in tobacco-growing soils.
Methods: After 30,000 kg·hm⁻² overpressure and 28 days of natural decomposition, plants (Hordeum vulgare L. (DM), Secale cereale L.
[A novel carbonyl reductase for the synthesis of ()-tolvaptan].
Sheng Wu Gong Cheng Xue Bao
January 2025
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry and Materials Sciences, Hebei University, Baoding 071002, Hebei, China.
Screening carbonyl reductases with the ability to catalyze the reduction of complex carbonyl compounds is of great significance for the biosynthesis of -tolvaptan(-TVP). In this study, the target carbonyl reductase in the crude enzyme extract of rabbit liver was separated, purified, and identified by ammonium sulfate precipitation, gel-filtration chromatography, ion exchange chromatography, affinity chromatography, and protein mass spectrometry. With the rabbit liver genome as the template, the gene encoding the carbonyl reductase was amplified by PCR and the recombinant strain was successfully constructed.
View Article and Find Full Text PDFRelationship assessment of microbial community and cometabolic consumption of 2-chlorophenol.
Appl Microbiol Biotechnol
January 2025
Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril San Rafael Atlixco 186, Col. Leyes de Reforma 1A Sección, Iztapalapa, CDMX, Mexico City, Mexico.
The relationship of microbial community and cometabolic consumption of 2-chlorophenol (2-CP) in a nitrifying sequencing batch reactor (SBR) was studied. The assessment of the population dynamics of the nitrifying sludge during the cometabolic 2-CP consumption with increasing ammonium (NH) concentrations in the SBR showed the presence of 39 different species of which 10 were always present in all cycles. Fifty-five percent of the species found were grouped as Proteobacteria (45% as β-proteobacteria and 10% as γ-proteobacteria class), 30% as Acidobacteria, and 15% as Deinococcus-Thermus phyla.
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