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Electrophoresis-Correlative Data-Independent Acquisition (Eco-DIA) Improves the Sensitivity of Mass Spectrometry for Limited Proteome Amounts.
Anal Chem
October 2024
Department of Chemistry & Biochemistry, University of Maryland, College Park, Maryland 20742, United States.
Capillary zone electrophoresis (CE) combines high separation power, scalability, and speed to limited proteome analyses by mass spectrometry (MS). However, compressed separation in CE challenges the duty cycle of tandem MS, even during data-independent acquisition (DIA). To help remedy this limitation, we introduce the concept of lectrophoresis-rrelative (Eco) data acquisition for CE-MS.
View Article and Find Full Text PDFRapid identification of antibody impurities in size-based electrophoresis via CZE-MS generated spectral library.
Sci Rep
August 2024
Johnson & Johnson Innovative Medicine Research & Development, 1400 McKean Road, Spring House, PA, 19477, USA.
Article Synopsis
- Reliable impurity detection in biopharmaceuticals is key for ensuring product quality and safety; however, traditional methods face technical challenges in accurately identifying individual impurities.
- This study introduces a size-based electrophoresis method combined with mass spectrometry to effectively detect and identify impurities in antibody production from known degradation products.
- By simulating cell culture conditions and forced degradation, the researchers demonstrate that this combined approach improves the detection and identification of impurities, enhancing the development process for antibody therapeutics.*
NC Meets CN: Porous Photoanodes with Polymeric Carbon Nitride/ZnSe Nanocrystal Heterojunctions for Photoelectrochemical Applications.
ACS Appl Mater Interfaces
July 2024
Department of Chemistry and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
The utilization of photoelectrochemical cells (PEC) for converting solar energy into fuels (e.g., hydrogen) is a promising method for sustainable energy generation.
View Article and Find Full Text PDFGold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer.
Biosensors (Basel)
March 2024
LAQV/REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua Campo Alegre, 687, 4169-007 Porto, Portugal.
Gold nanoparticles (AuNPs) exhibit improved optical and spectral properties compared to bulk materials, making them suitable for the detection of DNA, RNA, antigens, and antibodies. Here, we describe a simple, selective, and rapid non-cross linking detection assay, using approx. 35 nm spherical Au nanoprobes, for a common mutation occurring in exon 19 of the epidermal growth factor receptor (EGFR), associated with non-small-cell lung cancer cells.
View Article and Find Full Text PDFThe dyeing effect of acridine orange for multiple plasmid systems is sensitive to temperature.
J Cell Biochem
February 2024
Department of Pathogenic Biology, Chengdu Medical College, Chengdu, China.
The Goldview dyeing of the natural multiplasmid system of Lactobacillus plantarum PC518 was affected by temperature. The article want to identify the specific molecules that cause temperature sensitivity, then experiment on the universality of temperature sensitivity, and finally preliminarily analyze the influencing factors. At 5°C and 25°C, single pDNA, multiplasmid system, and linear DNA samples were electrophoretic on agarose gel prestained by Goldview 1, 2, 3, and acridine orange (AO), respectively.
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