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[Preparation and identification of monoclonal antibodies against human LAG3 by immunizing mice with recombinant eukaryotic cell antigens].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2024
Department of Medical Experimental Center, Northern Jiangsu People's Hospital, Yangzhou 225001, China. *Corresponding author, E-mail: yyue_king
Objective To prepare mouse anti-human lymphocyte activation gene 3 (LAG3) monoclonal antibody (mAb) and perform immunological identification of the antibody. Methods BALB/c mice were immunized with LAG3-mLumin-3T3 cells, which stably express the extracellular and transmembrane regions of human LAG3 in mouse 3T3 cells. The secretion of anti-human LAG3 antibodies in mouse serum was assessed using flow cytometry and immunofluorescence.
View Article and Find Full Text PDFAnthracene-Conjugated Steroidal Amphiphiles: Soft Functional Materials Exhibiting Supramolecular Aggregation Induced Enhanced Emission with Potential Applications as Drug Carriers and Fluorescent Bioprobes.
Langmuir
January 2025
Department of Chemistry, National Institute of Technology, Rourkela, Odisha 769008, India.
Bile salts (BS) are naturally occurring steroidal biosurfactants. The ease of functionalization of BSs has boosted their use as inexpensive building blocks for the fabrication of a broad set of value-added soft functional materials. In the present work, three fluorescent bile acid (FBA) derivatives have been synthesized by conjugating anthracene at the side chain of lithocholic acid, deoxycholic acid, and cholic acid to understand the effect of the nature of the steroid nucleus on their physicochemical properties.
View Article and Find Full Text PDFImproved bacterial cellulose production by Acetobacter oryzoeni MGC-N8819 in tobacco waste extract coupled with nicotine removal by Pseudomonas sp. JY-Q/5∆.
Int J Biol Macromol
December 2024
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, Zhejiang Province, PR China. Electronic address:
As the substrate, tobacco waste extract (TWE) can produce bacterial cellulose (BC), a biobased material. However, nicotine inhibits BC production (adding 0.8 g/L nicotine to the HS medium had a negative effect on BC synthesis) and needs to be removed.
View Article and Find Full Text PDFAntiviral activity of on HSV-1, 2 .
Iran J Microbiol
December 2024
Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Background And Objectives: Medicinal plants are the primary treatment for many infectious and non-infectious diseases. In this study, we evaluated the antiviral activity of against herpes simplex viruses 1 and 2, and compared it with the antiviral drug acyclovir.
Materials And Methods: In our experimental study, was dissolved in DMSO, then diluted in DMEM medium.
Production of ectoine by Vreelandella boliviensis using non-aseptic repeated-batch and continuous cultivations in an air-lift bioreactor.
Int Microbiol
December 2024
Center of Biotechnology, Faculty of Science and Technology, Universidad Mayor de San Simón, Cochabamba, Bolivia.
Ectoine, an osmolyte produced by various microorganisms, has numerous commercial applications. Vreelandella boliviensis (formerly called Halomonas boliviensis) generates high ectoine concentrations, i.e.
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