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Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess.
J Vis Exp
June 2022
Department of Anesthesiology, Columbia University Medical Center.
The mitochondrial permeability transition pore (mPTP) is a voltage-gated, nonselective, inner mitochondrial membrane (IMM) mega-channel important in health and disease. The mPTP mediates leakage of protons across the IMM during low-conductance opening and is specifically inhibited by cyclosporine A (CsA). Coenzyme Q (CoQ) is a regulator of the mPTP, and tissue-specific differences have been found in CoQ content and open probability of the mPTP in forebrain and heart mitochondria in a newborn mouse model of fragile X syndrome (FXS, Fmr1 knockout).
View Article and Find Full Text PDFAnalytic and chemometric assessments of the native probiotic bacteria and inulin effects on bioremediation of lead salts.
J Sci Food Agric
September 2021
Department of Food and Drug Control, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Background: Lead (Pb ) is one of the most toxic heavy metals and can be found in various quantities in the environment. The five native probiotic bacteria and inulin were used to assess in vitro lead nitrate and lead acetate binding capacities, as well as removal potentials.
Results: The highest decrease in media pH was seen for samples containing a combination of Lactobacillus paracasei IRBC-M 10784, lead nitrate and inulin (5.
Probing the role of arginine 323 of the D1 protein in photosystem II function.
Physiol Plant
February 2021
I2BC, UMR CNRS 9198, CEA Saclay, Saclay, France.
The Mn CaO cluster of photosystem II (PSII) advances sequentially through five oxidation states (S to S ). Under the enzyme cycle, two water molecules are oxidized, O is generated and four protons are released into the lumen. Umena et al.
View Article and Find Full Text PDFEffects of isoflurane on complex II‑associated mitochondrial respiration and reactive oxygen species production: Roles of nitric oxide and mitochondrial KATP channels.
Mol Med Rep
November 2019
Institute of Clinical Medicine Research, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, Jiangsu 215153, P.R. China.
Volatile anesthetics may protect the heart against ischemia‑reperfusion injury via the direct action on mitochondrial complexes and by regulating the production of reactive oxygen species (ROS). Recently, we reported that isoflurane induced the attenuation of mitochondrial respiration caused by complex I substrates. This process was not associated with endogenous production of mitochondrial nitric oxide (NO).
View Article and Find Full Text PDFPolarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected extracts and quinic acid, their antidiabetic ingredient.
Nat Prod Res
May 2021
Department of Life Sciences, Institute for Multidisciplinary Research - IMSI, University of Belgrade, Serbia.
This study was focused on screening of the total antioxidant activity of the selected extracts of the mushroom and quinic acid, one of their antidiabetic ingredients, by an uncommon electrochemical assay. Indeed, direct current (DC) polarographic HydroxoPerhydroxo Mercury(II) Complex (HPMC) assay based on decrease of anodic limiting current originating from HPMC formation in alkaline solutions of hydrogen peroxide at potential of mercury dissolution, observed upon gradual addition of antioxidants, was applied herein for the estimation of the natural products' antioxidativity. Quinic acid was found to exhibit most promising antioxidant potential (4.
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