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tRNA gene content, structure, and organization in the flowering plant lineage.
Front Plant Sci
December 2024
National Institute of Molecular Biology and Biotechnology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.
Transfer RNAs (tRNAs) are noncoding RNAs involved in protein biosynthesis and have noncanonical roles in cellular metabolism, such as RNA silencing and the generation of transposable elements. Extensive tRNA gene duplications, modifications to mature tRNAs, and complex secondary and tertiary structures impede tRNA sequencing. As such, a comparative genomic analysis of complete tRNA sets is an alternative to understanding the evolutionary processes that gave rise to the extant tRNA sets.
View Article and Find Full Text PDFThe mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition.
Nat Commun
December 2024
State Key Laboratory of Anti-Infective Drug Discovery and Development, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China.
The faithful charging of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases (AARSs) determines the fidelity of protein translation. Isoleucyl-tRNA synthetase (IleRS) distinguishes tRNA from tRNA solely based on the nucleotide at wobble position (N34), and a single substitution at N34 could exchange the aminoacylation specificity between two tRNAs. Here, we report the structural and biochemical mechanism of N34 recognition-based tRNA discrimination by Saccharomyces cerevisiae IleRS (ScIleRS).
View Article and Find Full Text PDFFormyl-methionine-mediated eukaryotic ribosome quality control pathway for cold adaptation.
Mol Cell
December 2024
Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea. Electronic address:
Protein synthesis in the eukaryotic cytosol can start using both conventional methionine and formyl-methionine (fMet). However, a mechanism, if such exists, for detecting and regulating the incorporation of fMet (instead of Met) during translation, thereby preventing cellular toxicity of nascent fMet-bearing (fMet-) polypeptides, remains unknown. Here, we describe the fMet-mediated ribosome quality control (fMet-RQC) pathway in Saccharomyces cerevisiae.
View Article and Find Full Text PDFAtTRM11 as a tRNA 2-methylguanosine methyltransferase modulates flowering and bacterial resistance via translational regulation.
Plant Sci
December 2024
College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China. Electronic address:
2-methylguanosine is an eukaryote-specific modified nucleoside in transfer RNAs, and mG10 is catalyzed by Trm11-Trm112 protein complex in eukaryotic tRNAs. Here, we show that loss-of-function mutation of the Arabidopsis Trm11 homolog AtTRM11 resulted in mG deficiency associated with disturbed ribosome assembly and overall transcriptome changes, including genes involved in flowering regulation and plant-pathogen interaction. The attrm11 mutant showed phenotypes of enlarged rosette leaves and early flowering, as well as enhanced resistance to Pseudomonas bacterial infection.
View Article and Find Full Text PDFMolecular basis for thiocarboxylation and release of Urm1 by its E1-activating enzyme Uba4.
Nucleic Acids Res
December 2024
Malopolska Centre of Biotechnology (MCB), Jagiellonian University, Gronostajowa7a, 30-387 Krakow, Poland.
Ubiquitin-related modifier 1 (Urm1) is a highly conserved member of the ubiquitin-like (UBL) family of proteins. Urm1 is a key component of the eukaryotic transfer RNA (tRNA) thiolation cascade, responsible for introducing sulfur at wobble uridine (U34) in several eukaryotic tRNAs. Urm1 must be thiocarboxylated (Urm1-SH) by its E1 activating enzyme UBL protein activator 4 (Uba4).
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