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Retrospective Comparison of Two Aspergillus IgG Enzyme Immunoassays for Diagnosing Pulmonary Aspergillosis.
Med Mycol J
September 2024
Department of Respiratory Medicine and Infectious Diseases, Niigata University Medical & Dental Hospital.
Article Synopsis
- Aspergillus-specific antibodies are used to diagnose allergic bronchopulmonary aspergillosis (ABPA) and chronic pulmonary aspergillosis (CPA), but tests for these were not commonly used in Japan.
- A study evaluated 64 patients with suspected ABPA and CPA using two different enzyme-linked immunosorbent assays (ELISAs) to measure serum Aspergillus IgG levels.
- Results showed that both the Bordier and Bio-Rad kits had high sensitivity and specificity for diagnosing CPA and ABPA, making them good alternatives to the discontinued precipitin test in clinical settings in Japan.
A human monoclonal antibody based immunoenzymetric assay to measure Fel d 1 concentrations in cat hair and pelt allergenic extracts.
Front Allergy
July 2024
Division of Allergy and Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
In the United States, 19 allergen extracts of different specificities are standardized, which means that their potencies are determined in comparison to a US reference standard. For cat allergen extracts, potency is determined by measuring Fel d 1 content expressed in in Fel d 1 units, and with a unitage that correlates with skin test reactions (bioequivalent allergy units or BAU). Currently, Fel d 1 content is measured with a radial immunodiffusion (RID) assay that uses polyclonal sheep antisera to detect the allergenic protein by producing a white precipitin line in agar gel.
View Article and Find Full Text PDFIsoelectric focusing followed by affinity immunoblotting to detect monoclonal free light chains in monoclonal gammopathies: Comparison with immunofixation electrophoresis and free light chain ratio.
Ann Clin Biochem
July 2024
Department of Internal Medicine, Haematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Background: Isoelectric focusing (IEF) is a method with an exquisite resolution, and coupled with affinity immunoblotting (AIB), it can provide superior sensitivity to detect monoclonal free light chains (FLC).
Methods: We tested the hypothesis that IEF/AIB is more sensitive and specific for monoclonal FLC detection in serum and urine samples than conventional methods, that is, electrophoresis (ELP), immunofixation (IF) and serum FLC ratio assessment. Investigation included 107 samples of 68 patients, among which 21 multiple myeloma patients were recently tested for minimal residual disease and 18 patients with AL amyloidosis.
Comparison of coccidioidal complement fixation and quantitative immunodiffusion serology at a reference laboratory.
Med Mycol
January 2024
University of California, Davis, Department of Medical Microbiology and Immunology, Davis, CA, USA.
The incidence of coccidioidomycosis continues to increase. The diagnosis frequently relies on non-invasive diagnostic testing with immunodiffusion and complement fixation (CF) testing the current gold standard. A direct comparison of quantitative immunodiffusion and CF for IgG antibodies has not been previously reported.
View Article and Find Full Text PDFEvaluation of laboratory and on-farm tests to estimate colostrum quality for dairy cows.
J Dairy Sci
December 2023
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany.
The objectives of this study were to evaluate different analytical methods to determine colostrum quality in dairy cattle, including one laboratory-based method (ELISA) and 4 on-farm tests. We hypothesized that the colostral IgG concentration using different analytical methods, such as ELISA (mg/mL), digital Brix refractometer (% Brix), colostrometer (specific gravity and mg/mL), an outflow funnel (seconds), and a lateral flow assay (mg/mL), were highly correlated with the reference method, radial immunodiffusion (RID; mg/mL) and would generate comparable results. Colostrum samples were collected from 209 Holstein Friesian cows on 2 commercial dairy farms in Germany.
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