Rainbow trout maintained on ascorbic acid deficient and three grades of ascorbic acid supplemented diets (160--1280 mg ascorbic acid pr kg feed) for a period of 53 weeks, were studied for effects on plasma lipid levels. Fish fed the diet with no ascorbic acid manifested lethargy, lordosis, scoliosis, internal hemorrhages and low body weight. With regard to blood properties, ascorbic acid deficient fish had low hematocrit and high plasma levels of triglycerides and cholesterol but low level of free fatty acids. Plasma level of free fatty acids was low also in fish fed the diet containing the highest amount of ascorbic acid.
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Reprod Med Biol
January 2025
Laboratory of Animal Reproduction, Graduate School of Agricultural Sciences Yamagata University Tsuruoka Japan.
Purpose: This study aimed to investigate the molecular mechanisms associated with chromosome segregation errors caused by intrinsic oxidative stress during in vitro oocyte maturation (IVM) using oocytes from -deficient (KO) mice.
Methods: Ovulated or in vitro matured cumulus-cells oocyte complexes (COCs) were collected from wild-type (WT) and KO mice and evaluated chromosome alignment, chromosome segregation, meiotic progression, and BUBR1 and REC8 protein expression levels.
Results: In 21% O IVM, the KO had significantly higher frequencies of chromosome misalignment and segregation errors compared to the WT, and they also reached Germinal Vesicle Break Down (GVBD) and M I stages peak earlier and showed a shorter M I stage residence time compared to the WT.
BioTechnologia (Pozn)
December 2024
Department of Biology, Eskişehir Technical University (ESTU), Tepebaşı/Eskişehir, Türkiye.
Background: The present study investigated the antioxidant, antimicrobial, and partial enzymatic properties of 52 thermophilic cyanobacteria isolates .
Materials And Methods: The DPPH scavenging method was applied to test the antioxidant potential of isolates' methanol extracts. Agar block diffusion and agar well diffusion methods were used to evaluate the antimicrobial activity and measured in milimeters.
Langmuir
January 2025
Institute of Nano Science and Technology, Knowledge City, Sector 81, SAS Nagar, Mohali, Punjab 140306, India.
Enzyme-instructed signal generation at liquid-liquid interfaces presents a novel strategy for controlling and detecting biochemical processes on macroscopic scales. Here, we explore the self-assembly and jamming of pillar[5]arene (P[5]A) derivatives at the oil-water interface via a copper-mediated "click" reaction, providing a versatile platform for generating observable signals. The formation of a pillar[5]arenes network at the droplet interface reduces interfacial tension, allowing droplets to adopt various nonequilibrium shapes based on the interfacial jamming process.
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January 2025
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
This study investigated the mechanisms employed by exogenous dopamine application in alleviating chilling injury in kiwifruits during storage at 1 °C for 120 days. Our results indicated that dopamine treatment at 150 µM alleviated chilling injury in kiwifruits during storage at 1 °C for 120 days. By 150 µM dopamine application, higher SUMO E3 ligase (SIZ1) and target of rapamycin (TOR) genes expression accompanied by lower poly(ADP-Ribose) polymerase 1 (PARP1) and sucrose non-fermenting 1-related kinase 1 (SnRK1) genes expression was associated with higher salicylic acid, ATP, NADPH and proline accumulation in kiwifruits during storage at 1 °C for 120 days.
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January 2025
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Debrecen, Egyetem tér 1, Debrecen, 4032, Hungary.
Hydrogen sulfide (HS) is an endogenous gasotransmitter with cardioprotective and antiviral effects. In this work, new cysteine-selective nucleoside-HS-donor hybrid molecules were prepared by conjugating nucleoside biomolecules with a thiol-activatable dithioacetyl group. 5'-Dithioacetate derivatives were synthesized from the canonical nucleosides (uridine, adenosine, cytidine, guanosine and thymidine), and the putative 5'-thio metabolites were also produced from uridine and adenosine.
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