In the present work a method is described to standardize each step of leukocyte capillary tube migration assay. The conclusions of our study are: natrium heparin can be used as anticoagulant; the use of calibrated microcapillaries is recommended; migration chambers must lay on a horizontal surface; results can be evaluated by a ocular for radial immunodiffusion from the 16th to 20th hour of migration.
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