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Nanobody-based indirect competitive ELISA for the detection of aflatoxin M1 in dairy products.
Sci Rep
January 2025
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Aflatoxin M1 (AFM1) is known to be carcinogenic, mutagenic, and teratogenic and poses a serious threat to food safety and human health, which makes its surveillance critical. In this study, an indirect competitive ELISA (icELISA) based on a nanobody (Nb M4) was developed for the sensitive and rapid detection of AFM1 in dairy products. In our previous work, Nb M4 was screened from a Bactrian-camel-immunized phage-displayed library.
View Article and Find Full Text PDFIdentification and characterization of ClAPRR2, a key candidate gene controlling watermelon stripe color.
Plant Sci
January 2025
Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China. Electronic address:
The stripe color of watermelon is a vital commercial trait and is the focus of attention of consumers and researchers. However, the genetic determinants of watermelon stripe color are incompletely understood. Based on the results of preliminary localization studies, we constructed a large-capacity F generation population (710 plants) using light-green striped ZXG1555 and green-striped Cream of Saskatchewan (COS) watermelon strains as parental lines for fine mapping.
View Article and Find Full Text PDFThe abiologically and biologically driving effects on organic matter in marginal seas revealed by deep learning-assisted model analysis.
Sci Total Environ
January 2025
Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 101408, China. Electronic address:
The biogeochemical processes of organic matter exhibit notable variability and unpredictability in marginal seas. In this study, the abiologically and biologically driving effects on particulate organic matter (POM) and dissolved organic matter (DOM) were investigated in the Yellow Sea and Bohai Sea of China, by introducing the cutting-edge network inference tool of deep learning. The concentration of particulate organic carbon (POC) was determined to characterize the status of POM, and the fractions and fluorescent properties of DOM were identified through 3D excitation-emission-matrix spectra (3D-EEM) combined parallel factor analysis (PARAFAC).
View Article and Find Full Text PDFChallenges of Investigating Compartmentalized Brain Energy Metabolism Using Nuclear Magnetic Resonance Spectroscopy in vivo.
Neurochem Res
January 2025
Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden.
Brain function requires continuous energy supply. Thus, unraveling brain metabolic regulation is critical not only for our basic understanding of overall brain function, but also for the cellular basis of functional neuroimaging techniques. While it is known that brain energy metabolism is exquisitely compartmentalized between astrocytes and neurons, the metabolic and neuro-energetic basis of brain activity is far from fully understood.
View Article and Find Full Text PDFPopulation structure and genetic diversity of Toona sinensis revealed by whole-genome resequencing.
BMC Genom Data
January 2025
Key Laboratory of State Forestry and Grassland Administration Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm Resources, Ji'nan, 250103, Shandong, China.
Objectives: Toona sinensis, commonly known as Chinese toon, is a perennial woody plant with significant economic and ecological importance. This study employed whole-genome resequencing of 180 T. sinensis samples collected from Shandong to analyze genetic variation and diversity, ultimately identifying 18,231 high-quality SNPs after rigorous quality control and linkage disequilibrium pruning.
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