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Rho1 and other GTP-binding proteins are associated with vesicles carrying glucose oxidase activity from Fusarium oxysporum f. sp. lycopersici.
Antonie Van Leeuwenhoek
March 2011
División de Ciencias Naturales y Exactas, Departamento de Biología, Campus Guanajuato, Universidad de Guanajuato, Apdo postal 187, 36000 Guanajuato, Gto, Mexico.
In eucaryotic cells, the delivery of a secreted protein to the plasma membrane via vesicles must include transport, recognition, and fusion events. Proteins exposed on the cytoplasmic face of the secretory vesicles play a role in these events; these include the GTP-binding proteins, which are crucial components in this process. Fractions enriched with vesicles carrying glucose oxidase (GOX) activity from Fusarium oxysporum f.
View Article and Find Full Text PDFSmall GTP-binding proteins are associated with chitosomes and vesicles carrying glucose oxidase from Mucor circinelloides.
Microbiology (Reading)
March 2008
Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Apdo. postal 187, Guanajuato, Gto. 36000, Mexico.
Fractions enriched with chitosomes and vesicles carrying glucose oxidase (GOX) activity from the dimorphic zygomycete Mucor circinelloides were obtained using two successive sucrose gradients, the first a linear-log and the second an isopycnic gradient. Using an [alpha-(32)P]GTP-binding assay, we detected the association of small GTP-binding proteins (21 and 17 kDa) with both types of vesicles. In addition, by ADP-ribosylation with C3 exotoxin, and Western blot analysis with specific antibodies, we identified the small GTPases RhoA (Rho1p) and Rab8, and a 17 kDa protein, with pI values of 6.
View Article and Find Full Text PDFHigh purity fatty acid synthetase mRNA has been prepared from rat liver. The translational purity of the mRNA preparation was at least 27% as judged by the percentage of the radioactivity incorporated into acid-insoluble material that was precipitated by anti-fatty acid synthetase antibody. The specific activity of the mRNA was 220-times greater than that reported previously from this laboratory [1].
View Article and Find Full Text PDFPoly(A)-rich RNA was isolated from developing soybean seeds (Glycine max (L.) Merr.) and fractionated on linear log sucrose gradients.
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