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Autoinducer-2 detection among commensal oral streptococci is dependent on pH and boric acid.
J Microbiol
July 2016
School of Math and Sciences, Lincoln Memorial University, Harrogate, TN, USA.
Autoinducer-2, considered a universal signaling molecule, is produced by many species of bacteria; including oral strains. Structurally, autoinducer-2 can exist bound to boron (borated autoinducer-2). Functionally, autoinducer-2 has been linked to important bacterial processes such as virulence and biofilm formation.
View Article and Find Full Text PDFDetermination of autoinducer-2 in biological samples by high-performance liquid chromatography with fluorescence detection using pre-column derivatization.
J Chromatogr A
September 2014
Department of Chemistry, University of Science & Technology of China, Hefei 230026, China. Electronic address:
Autoinducer-2 (AI-2), as a small-molecular-weight organic molecule secreted and perceived by various bacteria, enables intra- and inter-species communications. Quantitative determination of AI-2 is essential for exploring the bacterial AI-2-related physiological and biochemical processes. However, current strategies for sensitive detection of AI-2 require sophisticated instruments and complicated procedures.
View Article and Find Full Text PDFIntra-species bacterial quorum sensing studied at single cell level in a double droplet trapping system.
Int J Mol Sci
May 2013
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
In this paper, we investigated the intra-species bacterial quorum sensing at the single cell level using a double droplet trapping system. Escherichia coli transformed to express the quorum sensing receptor protein, LasR, were encapsulated in microdroplets that were positioned adjacent to microdroplets containing the autoinducer, N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL). Functional activation of the LasR protein by diffusion of the OdDHL across the droplet interface was measured by monitoring the expression of green fluorescent protein (GFP) from a LasR-dependent promoter.
View Article and Find Full Text PDFScrambling of autoinducing precursor peptides investigated by infrared multiphoton dissociation with electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry.
Anal Bioanal Chem
February 2013
Dipartimento di Scienze, Università degli Studi della Basilicata, Potenza, Italy.
Two synthetic precursor peptides, H(2)N-CVGIW and H(2)N-LVMCCVGIW, involved in the quorum sensing of Lactobacillus plantarum WCFS1, were characterized by mass spectrometry (MS) with electrospray ionization and 7-T Fourier transform ion cyclotron resonance (ESI-FTICR) instrument. Cell-free bacterial supernatant solutions were analyzed by reversed-phase liquid chromatography with ESI-FTICR MS to verify the occurrence of both pentapeptide and nonapeptide in the bacterial broth. The structural characterization of both protonated peptides was performed by infrared multiphoton dissociation using a continuous CO(2) laser source at a wavelength of 10.
View Article and Find Full Text PDFControlling the contents of microdroplets by exploiting the permeability of PDMS.
Lab Chip
March 2011
Department of Chemistry, University of Cambridge, Cambridge, UK.
A microfluidic device capable of exploiting the permeability of small molecules through polydimethylsiloxane (PDMS) has been fabricated in order to control the contents of microdroplets stored in storage wells. We demonstrate that protein precipitation and crystallization can be triggered by delivery of ethanol from a reservoir channel, thus controlling the protein solubility in microdroplets. Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) through the PDMS membrane of the device.
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