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Fluorometric detection of phages in liquid media: Application to turbid samples.
Anal Chim Acta
May 2020
Departament de Genètica I de Microbiologia, Universitat Autònoma de Barcelona. Edifici C, Campus de Bellaterra, 08193, Cerdanyola Del Vallès, Barcelona, Spain. Electronic address:
During the last years there has been a growing interest in the development of methods for phage detection and quantification in environmental, public health and industrial sectors. Good methods of phage monitoring contribute to progress in phage therapies, biocontrol and food safety studies. They have also been used to indicate the possible presence of microbiological hazards in drinking and recreational waters, and are an essential tool to prevent failure of microbe-based industrial bioreactors.
View Article and Find Full Text PDFQuantification and mapping of antigenic determinants of serum amyloid A (SAA) protein utilizing sequence-specific immunoglobulins and Eu3+ as a specific probe for time-resolved fluorometric immunoassay.
J Immunol Methods
May 1995
Karl-Franzens University Graz, Institute of Medical Biochemistry, Austria.
Serum amyloid A (SAA) protein, the most prominent amongst acute-phase proteins, is the specific precursor protein of secondary reactive amyloidosis. The fact that SAA once released into the circulation as a 'free' protein rapidly associates with lipoproteins of the high-density range indicates a specific role in lipoprotein metabolism. In this study a new sensitive assay for quantification of human SAA protein in biological specimens using affinity-purified polyclonal antibodies and Eu3+ as a specific probe for time-resolved fluorometric immunoassay is presented.
View Article and Find Full Text PDFThe concentration of IgA in a serum was 5.99 g/L as assayed nephelometrically with reagent from one company, but varied between 5 and 3 g/L (for sixfold and 36-fold dilutions, respectively) without giving a definitive answer when assayed with reagent from another source. Immunofixation electrophoresis indicated an IgA lambda monoclonal protein of 45 g/L.
View Article and Find Full Text PDFIncrease of serum angiotensin-converting enzyme activity after freezing.
Scand J Clin Lab Invest
September 1984
A fluorometric assay [4] of angiotensin-converting enzyme (ACE) was adapted to a new fluorometric and nephelometric Transcon 102 FN analyser. The intraassay coefficient of variation for the method was 2.6% (n = 48).
View Article and Find Full Text PDFNephelometric measurement of light-scattering index and visual estimates of turbidity have been advocated to monitor serum Intralipid levels. This study describes a simple modified fluorometric method for accurately measuring lipid particles in serum and examines the reliability of such estimates compared with other chemical measurements. Ten percent IL was diluted with either saline or serum to various concentrations (0 to 250 mg/dl).
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