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Expression and Characterization of Alkaline Phosphatase from KMM 296 in Transiently Transformed Tobacco Leaves and Transgenic Calli.
Plants (Basel)
December 2024
School of Advanced Engineering Studies, Institute of Biotechnology, Bioengineering and Food Systems, FEFU, 10 Ajax Bay, 690922 Vladivostok, Russia.
Alkaline phosphatase (ALP) of the PhoA family is an important enzyme in mammals, microalgae, and certain marine bacteria. It plays a crucial role in the dephosphorylation of lipopolysaccharides (LPS) and nucleotides, which overstimulate cell signaling pathways and cause tissue inflammation in animals and humans. Insufficient ALP activity and expression levels have been linked to various disorders.
View Article and Find Full Text PDFMolecular Characteristics of Community-Acquired Methicillin-Resistant , Hospital-Acquired MRSA Isolates, and PVL in one of the Indian hospitals.
Indian J Microbiol
December 2024
Department of Internal Medicine, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, 160012 India.
Unlabelled: Community-acquired methicillin resistant (CA-MRSA) strains are increasingly replacing hospital-acquired MRSA (HA-MRSA) strains in hospitalized patients leading to poor clinical outcomes. Hence, this study aimed to characterize clinical isolates of MRSA (HA-MRSA and CA-MRSA) and to understand their clonal origin. A total of 400 consecutive clinical isolates were collected from the clinical bacteriology lab of a tertiary care hospital.
View Article and Find Full Text PDFMicrofluidic Dielectrophoretic Purification of Extracellular Vesicles from Plasma Lipoproteins.
Langmuir
December 2024
Center for Algorithmic and Robotized Synthesis, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.
Extracellular vesicles (EVs) are small lipid vesicles shed by cells, carrying proteins, nucleic acids, and other molecular fingerprints. EVs have emerged as crucial mediators of cell-to-cell communication and hold great promise as biomarkers for liquid biopsies, enabling disease screening, diagnosis, prognosis, and monitoring. However, conventional EV separation methods are hampered by the presence of lipoproteins (LPs) in plasma samples, which have comparable characteristics and significantly outnumber EVs.
View Article and Find Full Text PDFMethicillin-Resistant Carrying the PVL and Toxic Shock Syndrome Toxin in Healthy Dogs in Algeria.
Antibiotics (Basel)
November 2024
Unité Antibiorésistance et Virulence Bactériennes, ANSES-Université de Lyon, 69007 Lyon, France.
: and are major opportunistic pathogens in both humans and dogs. In pets, the dissemination of methicillin-resistant isolates (MRSA or MRSP) is problematic for the treatment of animals and is a public health issue due to their zoonotic potential. MRSA and MRSP may also harbor virulent genes that increase their dangerousness.
View Article and Find Full Text PDFBe bold, start cold! cold formalin fixation of colorectal cancer specimens granted superior DNA and RNA quality for downstream molecular analysis.
Histochem Cell Biol
December 2024
Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy.
The use of cold formalin fixation (CFF; i.e., fixating tissue samples with 4 °C precooled formalin) recently attracted further attention owing to its putative improved ability to preserve nucleic acid compared with standard room temperature formalin (SFF).
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