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An integrated immunofluorescent detection system for automated and sensitive protein quantification based on a microfluidic flow cytometry platform.
Anal Chim Acta
March 2025
Holosensor Medical Technology Ltd, Room 12, No. 1798, Zhonghuayuan West Road, Yushan Town, Suzhou, 215000, China; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK. Electronic address:
Rapid and sensitive protein detection methods are of benefit to clinical diagnosis, pathological mechanism research, and infection prevention. However, routine protein detection technologies, such as enzyme-linked immunosorbent assay and Western blot, suffer from low sensitivity, poor quantification and labourious operation. Herein, we developed a fully automated protein analysis system to conduct fast protein quantification at the single molecular level.
View Article and Find Full Text PDFRoughness Impact of Piezoelectric Dental Scaler on Two Distinct Flowable Composite Filling Materials.
J Vis Exp
January 2025
Faculty of Dentistry, Department of Periodontology, Marmara University;
Dental ultrasonic scalers are commonly employed in periodontal treatment; however, their ability to roughen tooth surfaces is a worry since roughness may increase plaque production, a key cause of periodontal disease. This research studied the influence of a piezoelectric ultrasonic scaler on the roughness of two distinct flowable composite filling materials. To do this, 10 disc-shaped samples were generated from each of the two flowable composite materials.
View Article and Find Full Text PDFEvaluation by Scanning Electron Microscopy and Energy Dispersive X-Ray Spectroscopy of the Effects of Root Canal Instrumentation on the Radicular Dentine.
Microsc Res Tech
January 2025
Faculty of Dental Medicine, Grigore T. Popa University of Medicine and Pharmacy, Iasi, Romania.
One area of technological advancement has been the shift from stainless steel hand tools to nickel-titanium (Ni-Ti) rotary tools. This paper aims to perform an in vitro comparative study to evaluate the efficacy of five endodontic manual and rotary instruments such as Kerr files, Orodeka Plex V, ProTaper Flydent NiTi super files, and ProTaper Flydent NiTi super files in combination with an ultrasonic endodontic E3D Diamantata EMS scaler used for root canal shaping. The following aspects were highlighted: effective removal of smear layer (SL) from the dentinal tubules in the coronal 1/3, middle 1/3, and apical 1/3 of the root canal, appearance of cracks in the dentinal walls by SEM analysis, and highlighting of dentin mineral content and remnant debris by EDX analysis.
View Article and Find Full Text PDFInfective endocarditis following transcatheter mitral valve-in-valve replacement: a clinical case report.
BMC Cardiovasc Disord
January 2025
Department of Medical Ultrasonics, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Background: Transcatheter valve-in-valve replacement (TMViVR) is an alternative option for patients with bioprosthetic valve failure (BVF) who are at high surgical risk. Although infective endocarditis (IE) after transcatheter mitral valve-in-valve replacement is unusual, it is associated with significantly high mortality.
Case Presentation: An 81-year-old male patient was admitted with intermittent thoracic tightness, chest pain persisting for 3 years, and shortness of breath with nausea for 1 week.
Addressing residual and recurrent aneurysms post-Woven EndoBridge device embolization: a systematic review and meta-analysis.
J Neurointerv Surg
January 2025
Department of Neurosurgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, Buffalo, New York, USA
Background: Early literature on the Woven EndoBridge (WEB) device reported 80-90% adequate aneurysm occlusion but low complete occlusion (40-55%). It is uncertain whether residual or recurrent aneurysms require re-treatment to prevent future rupture.
Objective: To systematically review the literature to meta-analyze occlusion and complication rates after re-treatment of these aneurysms.
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