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Enhancing the physical and oxidative stability of hempseed protein emulsion via comparative enzymolysis with different proteases: Interfacial properties of the adsorption layer.
Food Res Int
February 2025
College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address:
Effects of enzymolysis by seven proteases (Alcalase, Bromelain, Flavourzyme, Papain, Pepsin, Protamex, and Trypsin) with distinct cleavage specificities on the emulsification performance of hempseed protein (HPI) and its correlation with the structural and interfacial characteristics were explored in this study. Upon enzymolysis, a remarkable decrease in α-helix and β-turn was observed in resultant hydrolysates (HPH), accompanied by a rise in β-sheet and random coil, notably by Alcalase, Bromelain, Papain, and Trypsin. Overall, proteolysis led to noticeable reductions in surface hydrophobicity and total sulfhydryls as well as a redshift in intrinsic fluorescence, with Papain showing the most pronounced effects, possibly due to its higher hydrolysis degree (4.
View Article and Find Full Text PDFHigh-throughput screening of dual-target inhibitors for SARS-CoV-2 main protease and papain-like protease from Chebulae Fructus: prediction and experimental verification.
Front Microbiol
December 2024
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Background: The unavoidable propagation of the coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has underscored the urgent requirement for efficacious therapeutic agents. The dried fruit of Retz., namely Chebulae Fructus, is widely used for treating bacterial and viral infectious diseases, which was witnessed to perform anti-SARS-CoV-2 activity in recommended Chinese patent medicine.
View Article and Find Full Text PDFEnhancing Digestibility and Intestinal Peptide Release of Protein: An Enzymatic Approach.
J Fungi (Basel)
December 2024
College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China.
is a tasty and low-calorie mushroom containing abundant high-quality protein. This study aims to improve the digestibility of protein (PEP) and hence to facilitate its development as a healthy alternative protein. The extracted PEP was pretreated with 1000-5000 U of papain, neutral protease and alkaline protease.
View Article and Find Full Text PDFA cell-based Papain-like Protease (PLpro) activity assay for rapid detection of active SARS-CoV-2 infections and antivirals.
PLoS One
December 2024
Mesa Photonics, Santa Fe, NM, United States of America.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants are a continuous threat to human life. An urgent need remains for simple and fast tests that reliably detect active infections with SARS-CoV-2 and its variants in the early stage of infection. Here we introduce a simple and rapid activity-based diagnostic (ABDx) test that identifies SARS-CoV-2 infections by measuring the activity of a viral enzyme, Papain-Like protease (PLpro).
View Article and Find Full Text PDFPreparation and characterization of acetylated starch/papain composites.
RSC Adv
November 2024
School of Chemical Engineering, Institute of Engineering, Suranaree University of Technology Nakhon Ratchasima 30000 Thailand
Article Synopsis
- - The research focused on creating and analyzing acetylated starch/papain composites by using a specific method to encapsulate papain in acetylated cassava starch, with a low degree of substitution (DS = 0.037).
- - Results showed that increasing starch concentration improved encapsulation efficiency (EE) but decreased loading capacity (LC) due to aggregation, while surfactants like Tween 80 enhanced EE and LC but reduced papain activity.
- - The best results were found with a 30 mg/mL starch concentration, 7 mL solution volume, and 3% v/v Tween 80, achieving a high EE of 96.23% but lowering papain activity to 56%; however
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