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Pancreatic Extracts for the Treatment of Diabetes (1889-1914): Acomatol.
Am J Ther
June 2020
Fundación DIABEM, Barcelona, Spain.
Background: Historical review on the early development of organotherapy for diabetes [pancreatic extracts (PE)] and its relationship with the social and political circumstances.
Areas Of Uncertainty: The diagnosis of diabetes relied only in the presence of glycosuria and cardinal symptoms. Blood glucose determinations were not regularly available, requiring large volumes for sampling.
Pork meat as a potential source of Salmonella enterica subsp. arizonae infection in humans.
J Clin Microbiol
March 2014
Laboratory of Microbiology and Parasitology, Faculty of Veterinary Medicine, School of Health Science, University of Thessaly, Karditsa, Greece.
Salmonella enterica subsp. arizonae was isolated from 13 of 123 slaughtered pigs in central Greece. The samples cultured were feces, ileum tissue, mesenteric lymph nodes, and gallbladder swabs.
View Article and Find Full Text PDFSelective screening for lysosomal storage diseases with dried blood spots collected on filter paper in 4,700 high-risk colombian subjects.
JIMD Rep
August 2013
Centro de Investigaciones en Bioquimica (CIBI), Departamento de Ciencias Biologicas, Universidad de Los Andes, Carrera 1 No. 18A - 12 of. M-302, Bogota, Colombia,
Lysosomal storage disorders (LSDs) are a very heterogeneous group of hereditary disorders. The diagnostic process usually involves complex sampling, processing, testing, and validation procedures, performed by specialized laboratories only, which causes great limitations in reaching a diagnosis for patients affected by these diseases.There are few studies about LSDs in Colombia.
View Article and Find Full Text PDFPolymer nanofibre junctions of attolitre volume serve as zeptomole-scale chemical reactors.
Nat Chem
April 2009
Center for Photochemical Sciences and Department of Chemistry, Bowling Green State University, Bowling Green, Ohio 43403, USA.
Methods allowing chemical reactions to be carried out on ultra-small scales in a controllable fashion are potentially important for a number of disciplines, including molecular electronics, photonics and molecular biology, and may provide fundamental insight into chemistry in confined spaces. Ultra-small-scale reactions also circumvent potential problems associated with reagent and product toxicity, and reduce energy consumption and waste generation. Here, we report a technique for performing chemical reactions on a zeptomole (10(-21) mol) scale.
View Article and Find Full Text PDFIdentification of enzymes and activity from two-dimensional gel electrophoresis.
Nat Protoc
January 2008
Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria.
Identification of proteins with enzymatic activity by mass spectrometry (MS) and concomitant determination of function by screening enzyme activity from two-dimensional gel electrophoresis (2DE) is one of the challenges of gel-based proteomics. In this protocol, proteins are extracted from spinal cord tissue followed by 2DE with in-gel digestion and identification by matrix-assisted laser desorption/ionization. Protein spots identified as possible enzyme of interest are punched, eluted by SDS-containing Tris buffer and renatured by buffers under reductive conditions.
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