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Introduction: Varicella-Zoster virus (VZV) is a highly contagious alpha-herpes virus. The diagnosis of chickenpox remains a difficult task especially in cases of breakthrough chickenpox, so the development of reliable laboratory tests is necessary. The simplest and most sensitive serological test for detecting antibodies in human and animal sera is the passive hemagglutination reaction (PHAR).
View Article and Find Full Text PDFRapid detection assays for Bacillus anthracis, Yersinia pestis, and Brucella spp. via triplex-recombinase polymerase amplification.
Mol Biol Rep
January 2025
State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China.
Background: Bacillus anthracis (B. anthracis), Yersinia pestis (Y. pestis), and Brucella spp.
View Article and Find Full Text PDFThe Relationships Between Body-Related Emotion Intolerance and Restrictive Eating as a Function of Multidimensional Perfectionism.
J Clin Psychol
January 2025
Department of Psychological Clinical Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada.
Objective: Emotion intolerance and perfectionism are two maintaining mechanisms to eating disorder symptomology. However, it is unclear how these mechanisms relate to one another. This study explored whether perfectionism is a vulnerability factor for facets of restrictive eating in the context of body-related emotions.
View Article and Find Full Text PDFPlasmid Library Construction From Genomic DNA.
Curr Protoc
January 2025
New England Biolabs, Ipswich, Massachusetts.
Functional genomic approaches have been effective at uncovering the function of uncharacterized genes and identifying new functions for known genes. Often these approaches rely on an in vivo screen or selection to associate genes with a phenotype of interest. These selections and screens are dependent upon the expression of proteins encoded in genomic DNA from an expression vector, such as a plasmid.
View Article and Find Full Text PDFEntropy-based methods for formulating bottom-up ultra-coarse-grained models.
J Chem Phys
January 2025
Department of Chemistry, Chicago Center for Theoretical Chemistry, James Franck Institute, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA.
Bottom-up coarse-grained (CG) modeling is an effective means of bypassing the limited spatiotemporal scales of conventional atomistic molecular dynamics while retaining essential information from the atomistic model. A central challenge in CG modeling is the trade-off between accuracy and efficiency, as the inclusion of often pivotal many-body interaction terms in the CG force-field renders simulation markedly slower than simple pairwise models. The Ultra Coarse-Graining (UCG) method incorporates many-body terms through discrete internal state variables that modulate the CG force-field according to, e.
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