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Dynamic changes of heterogeneous cell wall macromolecules in differentiating conifer xylem using cytochemical localization.
Int J Biol Macromol
January 2025
Department of Wood Anatomy and Utilization, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China; Wood Specimen Resource Center (WOODPEDIA) of National Forestry and Grassland Administration, Beijing 100091, China. Electronic address:
Article Synopsis
- Understanding the changes in cell wall components during xylem differentiation is crucial for grasping the structure of wood, especially at the secondary cell wall layer level.
- In the study of Pinus bungeana, it was found that crystalline cellulose and glucomannan were deposited before xylan and lignin, with lignification starting in the primary cell wall corners as the S layer developed.
- Immunofluorescence results showed that glucomannan was deposited earlier than xylan, and while pectins were concentrated in the primary walls, they decreased as tracheid walls differentiated, with ray cells lagging behind axial tracheids in their development.
Comparative electron microscopic visualization of the lung alveolar epithelial glycocalyx with different staining and labeling methods.
J Anat
September 2024
Institute of Functional Anatomy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Article Synopsis
- * The study aimed to improve visualization techniques for this glycocalyx using chemical fixation and various staining methods in mice, comparing agents like alcian blue, ruthenium red, and lanthanum nitrate.
- * Results showed that alcian blue provided the best staining, while the study's methods also align with animal welfare principles by promoting reduction and refinement in the use of animals for research.
Promotion of Bone Formation in a Rat Osteoporotic Vertebral Body Defect Model via Suppression of Osteoclastogenesis by Ectopic Embryonic Calvaria Derived Mesenchymal Stem Cells.
Int J Mol Sci
July 2024
Department of Neurosurgery, CHA Bundang Medical Center, CHA University, Seongnam-si 13496, Republic of Korea.
Osteoporotic vertebral compression fractures (OVCFs) are the most prevalent fractures among patients with osteoporosis, leading to severe pain, deformities, and even death. This study explored the use of ectopic embryonic calvaria derived mesenchymal stem cells (EE-cMSCs), which are known for their superior differentiation and proliferation capabilities, as a potential treatment for bone regeneration in OVCFs. We evaluated the impact of EE-cMSCs on osteoclastogenesis in a RAW264.
View Article and Find Full Text PDFThe Battle Against Quenching and Fading for Fluorescence: A Model of How to Evaluate the Fluorophore's Behavior.
J Histochem Cytochem
July 2023
Maryland Psychiatric Research Center, Department of Psychiatry, University of Maryland, Catonsville, Maryland.
The fading and quenching of fluorescence intensity has been a major problem in the use of fluorescein isothiocyanate (FITC) for immunofluorescence cytochemical techniques, especially with laser confocal microscopy. The companion article by Longin et al. provided an empirical approach to overcoming this problem.
View Article and Find Full Text PDFThe application of alcian blue to identify astrocyte-associated amyloid plaques by using fluorescence and confocal microscopy.
J Neurosci Methods
March 2023
Institute of Experimental Medicine, St. Petersburg 197376, Russia.
Background: Astrocytes play an essential role in the normal functioning of the nervous system and are active contributors to the pathogenesis of neurodegenerative diseases such as Alzheimer's disease (AD). Therefore, to comprehend the astrocytes and amyloid plaques relationship there is a need for imaging techniques providing simultaneous visualization of astrocytes using fluorescence and amyloid plaques revealed by transmitted light microscopy.
New Method: The possibility of simultaneous detection of astrocytes by immunocytochemistry (fluorescent) and amyloid plaques by cytochemical Alcian Blue (transparent) using confocal microscopy in 8-month-old 5хFAD mice samples shown.
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