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HPTLC Combined with sHetCA and Multivariate Statistics for the Detection of Bioactive Compounds in Complex Mixtures.
Molecules
December 2024
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
High-Performance Thin Layer Chromatography (HPTLC) is widely utilized in natural products research due to its simplicity, low cost, and short total analysis time, including data treatment. While bioautography can be used for rapid detection of bioactive compounds in extracts, the number of available bioautographic methods is limited mainly due to the high cost and difficulty in developing protocols that lead to accurate and reproducible results. For this reason, an alternative method for the detection of bioactive compounds in plant extracts prior to their isolation using HPTLC, combined with multivariate chemometrics, was previously explored by our lab.
View Article and Find Full Text PDFIdentification of pancreatin inhibitors from Thai medicinal Piper plants for antidiabetic and anti-obesity activities using high-performance thin-layer chromatography-bioautographic assay.
J Chromatogr A
November 2024
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Natural Products and Nanoparticles, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address:
Exploring the potential of natural products against diabetes and obesity is in demand nowadays. Pancreatic α-amylase and pancreatic lipase are the drug targets to minimize the absorption of glucose from starch and fatty acids from lipids, respectively. In this study, five Piper species, namely P.
View Article and Find Full Text PDFImpact of ultra-high dilutions from Iranian endemic and commercial on the germination and growth quality of L.
Heliyon
August 2024
Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, 1983969411, Tehran, Iran.
Purpose: This article deals with producing ultra-diluted compounds (UHDs) prepared from Iranian calendula's characteristic and endemic species. It compares their chemical, biological and biochemical characteristics with the commercial sample of calendula species (grown in the Alps). In the following, these UHDs have been used to improve the quality of germination and growth and reduce contamination of rice () seeds in the laboratory environment.
View Article and Find Full Text PDFTLC-MS-Bioautographic Identification of Antityrosinase Compounds and Preparation of a Topical Gel Formulation from a Bioactive Fraction of an RSM-Optimized Alcoholic Extract of Rubia Cordifolia L. stem.
J AOAC Int
November 2023
Jamia Hamdard, Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, New Delhi 110062, India.
Background: Rubia cordifolia L., Rubiaceae, is globally reported to treat skin-related problems. The study aimed to assess the antityrosinase potential of Rubia cordifolia (ARC) and the development of gel formulation.
View Article and Find Full Text PDFAntimicrobial properties and biotransforming ability of fungal endophytes from L. (Moraceae).
Mycology
February 2023
Farmacognosia, Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario-CONICET, Santa Fe, Argentina.
The endophytic fungal community associated with leaves of L. (Moraceae) from Argentina was investigated. Fifteen fungal isolates were isolated and identified by molecular methods into the genera and and were the most frequently isolated species.
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