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Short-Time Preamplification-Assisted One-Pot CRISPR Nucleic Acid Detection Method with Portable Self-Heating Equipment for Point-of-Care Diagnosis.
Anal Chem
January 2025
State Key Laboratory for Manufacturing Systems Engineering, School of Instrument Science and Techonology, Xi'an Jiaotong University, Xi'an 710054, China.
Infectious diseases, especially respiratory infections, have been significant threats to human health. Therefore, it is essential to develop rapid, portable, and highly sensitive diagnostic methods for their control. Herein, a short-time preamplified, one-pot clustered regularly interspaced short palindromic repeats (CRISPR) nucleic acid detection method (SPOC) is developed by combining the rapid recombinase polymerase amplification (RPA) with CRISPR-Cas12a to reduce the mutual interference and achieve facile and rapid molecular diagnosis.
View Article and Find Full Text PDFBackground: Population aging and the increase in memory-related diseases have motivated the search for accessible cognitive screening instruments. To develop a digital memory and learning test (DMLT) based on Rey's Auditory Verbal Learning Test (RAVLT) principles to assess cognition in the elderly and identify early cognitive decline.
Methods: The research was divided into two phases: developing the digital test and the experimental phase of comparison with a reference test.
Agreement analysis and associated factors of SARC-F and SARC-CALF in screening of risk sarcopenia in people living with human immunodeficiency virus.
Clinics (Sao Paulo)
January 2025
Posgraduate Program in Food, Nutrition and Health, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, Mato Grosso do Sul, Brazil. Electronic address:
Introduction: People Living with Human Immunodeficiency Virus (PLHIV) appear to be at a higher risk of developing sarcopenia. Various factors seem to influence the risk of sarcopenia, and its prevalence may differ depending on the screening tool used. This study aimed to (i) Screen the risk of sarcopenia in PLHIV using the SARC-F and SARCCalf and identify associated factors; (ii) Analyze the agreement between the instruments in PLHIV.
View Article and Find Full Text PDFRural patients' satisfaction with humanistic nursing care in Chinese Public Tertiary Hospitals: a national cross-sectional study.
Front Public Health
January 2025
Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, China.
Background: The provision of high-quality healthcare services and patient satisfaction are fundamental objectives in modern healthcare. Humanistic nursing care, which emphasizes empathy, respect for individuality, and cultural sensitivity, aims to build trust and improve the overall experience for patients. This approach is especially relevant for rural patients in China, who often face additional challenges in accessing care in large tertiary hospitals.
View Article and Find Full Text PDFPerceptions and Experiences of Surgical Nurses in Using the WHO Checklist in a Perioperative Setting: a Mixed-method Study.
Mater Sociomed
January 2024
Department of Anaesthesiology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Background: As a surgical nurse in a healthcare team, it is helpful to position the patient, depending on the nature of the procedure, and to prepare independently both the instrumentation and the site of surgery with drapes prior to the surgical procedure.
Objective: To examine the experience of surgical nurses in their work with the WHO surgical checklist in Sweden.
Methods: Data were collected from one hundred and ninety-six healthcare professionals, including thirty-nine surgical nurses, using a self-administered questionnaire.
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