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Expression and Characterization of Alkaline Phosphatase from KMM 296 in Transiently Transformed Tobacco Leaves and Transgenic Calli.
Plants (Basel)
December 2024
School of Advanced Engineering Studies, Institute of Biotechnology, Bioengineering and Food Systems, FEFU, 10 Ajax Bay, 690922 Vladivostok, Russia.
Alkaline phosphatase (ALP) of the PhoA family is an important enzyme in mammals, microalgae, and certain marine bacteria. It plays a crucial role in the dephosphorylation of lipopolysaccharides (LPS) and nucleotides, which overstimulate cell signaling pathways and cause tissue inflammation in animals and humans. Insufficient ALP activity and expression levels have been linked to various disorders.
View Article and Find Full Text PDFAn Epidemiological Study on in Tibetan Yaks from the Qinghai-Tibet Plateau Area in China.
Animals (Basel)
December 2024
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, China.
is an important foodborne pathogen that can cause a range of illnesses in humans; it has also been a key focus for monitoring in the field of public health, including gastroenteritis, sepsis, and arthritis, and can also cause a decline in egg production in poultry and diarrhea and abortion in livestock, leading to death in severe cases, resulting in huge economic losses. This study aimed to investigate the isolation rate, antimicrobial resistance, serotypes, and genetic diversity of isolated from yak feces in various regions on the Qinghai-Tibet Plateau. A total of 1222 samples of yak dung were collected from major cities in the Qinghai-Tibet Plateau area, and the sensitivity of the isolated bacteria to 10 major classes of antibiotics was determined using the K-B paper disk diffusion method for drug susceptibility.
View Article and Find Full Text PDFPrevalence, Distribution and Antimicrobial Susceptibility of Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain.
Environ Microbiol Rep
December 2024
Facultad de Ciencias de la Salud, Universidad Alfonso X el Sabio, Madrid, Spain.
Managing infections caused by multidrug-resistant Gram-negative bacilli is a major public health concern, particularly in hospitals where surfaces can act as reservoirs for resistant microorganisms. Identifying these bacteria in hospital environments is crucial for improving healthcare safety. This study aimed to analyse environmental samples from a veterinary hospital to identify prevalent microorganisms and detect antimicrobial resistance patterns.
View Article and Find Full Text PDFMolecular Characteristics of Community-Acquired Methicillin-Resistant , Hospital-Acquired MRSA Isolates, and PVL in one of the Indian hospitals.
Indian J Microbiol
December 2024
Department of Internal Medicine, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, 160012 India.
Unlabelled: Community-acquired methicillin resistant (CA-MRSA) strains are increasingly replacing hospital-acquired MRSA (HA-MRSA) strains in hospitalized patients leading to poor clinical outcomes. Hence, this study aimed to characterize clinical isolates of MRSA (HA-MRSA and CA-MRSA) and to understand their clonal origin. A total of 400 consecutive clinical isolates were collected from the clinical bacteriology lab of a tertiary care hospital.
View Article and Find Full Text PDFMicrofluidic Dielectrophoretic Purification of Extracellular Vesicles from Plasma Lipoproteins.
Langmuir
December 2024
Center for Algorithmic and Robotized Synthesis, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.
Extracellular vesicles (EVs) are small lipid vesicles shed by cells, carrying proteins, nucleic acids, and other molecular fingerprints. EVs have emerged as crucial mediators of cell-to-cell communication and hold great promise as biomarkers for liquid biopsies, enabling disease screening, diagnosis, prognosis, and monitoring. However, conventional EV separation methods are hampered by the presence of lipoproteins (LPs) in plasma samples, which have comparable characteristics and significantly outnumber EVs.
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