Electrophoreis of enterotoxin into a middle liquid section of a polyacrylamide gel column enhances recovery for serological assay.
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http://dx.doi.org/10.1128/am.18.6.1089-1090.1969 | DOI Listing |
Sovrem Tekhnologii Med
January 2025
MD, DSc, Professor, Chief Researcher, Laboratory of Molecular Genetic Testing of Therapeutic Diseases; Institution of Internal and Preventive Medicine - Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 175/1 B. Bogatkova St., Novosibirsk, 630089, Russia.
Unlabelled: was to search for the associations of benign unconjugated hyperbilirubinemia phenotype with rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) mutations of gene, rs113993960 (ΔF508) of gene, rs28929474 (PIZ), rs17580 (PIS) mutations of gene.
Material And Methods: The study design is case-control. The group with Gilbert's syndrome (GS) phenotype (n=414; mean age - 36.
J Pharm Biomed Anal
January 2025
Analytical Research and Development, Merck & Co., Inc., Rahway, NJ 07065, United States. Electronic address:
The dynamic landscape of mRNA technology highlights the need for innovative quality control (QC) strategies. In this study, we described an efficient one-step digestion approach for concurrent generation of 5'- and 3'-end fragments, enabling simultaneous mRNA capping and poly(A) tail analysis. Tailored 10-23-type DNAzymes, designed from 5'- and 3'-Untranslated Regions (UTRs), selectively cleaved mRNA to release both the 5'-Capped or uncapped short fragments and 3'-Poly(A) tail cleavage products.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, Thailand.
Direct thrombin inhibitors (designated as EuRL-DTIs) were partially purified from ethanol extracts of Euphorbia resinifera O.Berg latex. The obtained EuRL-DTIs comprised four major compounds: two isomers of phenolic compounds (CHO) and two amide compounds (tentatively identified as CHNO and CHNO), as identified by liquid chromatography and electrospray ionisation quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS), attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and/or nuclear magnetic resonance (NMR) spectroscopy.
View Article and Find Full Text PDFNucleic Acids Res
January 2025
Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697-3958, United States.
Growth in the development of engineered polymerases for synthetic biology has led to renewed interest in assays that can measure the fidelity of polymerases that are capable of synthesizing artificial genetic polymers (XNAs). Conventional approaches require purifying the XNA intermediate of a replication cycle (DNA → XNA → DNA) by denaturing polyacrylamide gel electrophoresis, which is a slow, costly, and inefficient process that requires a large-scale transcription reaction and careful extraction of the XNA strand from the gel slice. In an effort to streamline the assay, we developed a purification-free approach in which the XNA transcription and reverse transcription steps occur inside the matrix of a hydrogel-coated magnetic particle.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
College of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu, P.R. China.
Transitions between chiral rotational locomotion modes occur in a variety of active individuals and populations, such as sidewinders, self-propelled chiral droplets, and dense bacterial suspensions. Despite recent progress in the study of active matter, general principles governing rotational chiral transition remain elusive. Here, we study, experimentally and theoretically, rotational locomotion and its chiral transition in a 2D polyacrylamide (PAAm)-based BZ gel driven by Belousov-Zhabotinsky reaction-diffusion waves.
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