Electrophoreis of enterotoxin into a middle liquid section of a polyacrylamide gel column enhances recovery for serological assay.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC378199PMC
http://dx.doi.org/10.1128/am.18.6.1089-1090.1969DOI Listing

Publication Analysis

Top Keywords

polyacrylamide gel
8
technique rapid
4
rapid purification
4
purification entrapment
4
entrapment recovery
4
recovery enterotoxin
4
enterotoxin liquid
4
liquid chamber
4
chamber polyacrylamide
4
gel electrophoresis
4

Similar Publications

Unlabelled: was to search for the associations of benign unconjugated hyperbilirubinemia phenotype with rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) mutations of gene, rs113993960 (ΔF508) of gene, rs28929474 (PIZ), rs17580 (PIS) mutations of gene.

Material And Methods: The study design is case-control. The group with Gilbert's syndrome (GS) phenotype (n=414; mean age - 36.

View Article and Find Full Text PDF

DNAzyme approach for simultaneous mRNA cap and poly(A) tail length analysis: A one-step method to multiple quality attributes.

J Pharm Biomed Anal

January 2025

Analytical Research and Development, Merck & Co., Inc., Rahway, NJ 07065, United States. Electronic address:

The dynamic landscape of mRNA technology highlights the need for innovative quality control (QC) strategies. In this study, we described an efficient one-step digestion approach for concurrent generation of 5'- and 3'-end fragments, enabling simultaneous mRNA capping and poly(A) tail analysis. Tailored 10-23-type DNAzymes, designed from 5'- and 3'-Untranslated Regions (UTRs), selectively cleaved mRNA to release both the 5'-Capped or uncapped short fragments and 3'-Poly(A) tail cleavage products.

View Article and Find Full Text PDF

Direct thrombin inhibitors (designated as EuRL-DTIs) were partially purified from ethanol extracts of Euphorbia resinifera O.Berg latex. The obtained EuRL-DTIs comprised four major compounds: two isomers of phenolic compounds (CHO) and two amide compounds (tentatively identified as CHNO and CHNO), as identified by liquid chromatography and electrospray ionisation quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS), attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and/or nuclear magnetic resonance (NMR) spectroscopy.

View Article and Find Full Text PDF

Measuring XNA polymerase fidelity in a hydrogel particle format.

Nucleic Acids Res

January 2025

Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697-3958, United States.

Growth in the development of engineered polymerases for synthetic biology has led to renewed interest in assays that can measure the fidelity of polymerases that are capable of synthesizing artificial genetic polymers (XNAs). Conventional approaches require purifying the XNA intermediate of a replication cycle (DNA → XNA → DNA) by denaturing polyacrylamide gel electrophoresis, which is a slow, costly, and inefficient process that requires a large-scale transcription reaction and careful extraction of the XNA strand from the gel slice. In an effort to streamline the assay, we developed a purification-free approach in which the XNA transcription and reverse transcription steps occur inside the matrix of a hydrogel-coated magnetic particle.

View Article and Find Full Text PDF

Transitions between chiral rotational locomotion modes occur in a variety of active individuals and populations, such as sidewinders, self-propelled chiral droplets, and dense bacterial suspensions. Despite recent progress in the study of active matter, general principles governing rotational chiral transition remain elusive. Here, we study, experimentally and theoretically, rotational locomotion and its chiral transition in a 2D polyacrylamide (PAAm)-based BZ gel driven by Belousov-Zhabotinsky reaction-diffusion waves.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!