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Elucidating the life history parameters of Culicoides oxystoma (Diptera: Ceratopogonidae) under varied substrate compositions and temperatures.
J Med Entomol
January 2025
Entomology Research Unit, Department of Zoology, The University of Burdwan, Burdwan, India.
A comprehensive study was conducted on the life history parameters of an important vector Culicoides oxystoma Kieffer (Diptera: Ceratopogonidae), to standardize potential rearing procedures. Data on life history traits and rearing conditions are crucial for establishing laboratory colony and conducting vector competence-based studies utilizing specimens with a known rearing history. Six different substrate compositions were used to rear the larvae: S1: habitat mud containing cattle manure + nutrient broth + yeast, S2: yeast, S3: habitat mud containing cattle manure + nutrient broth, S4: nutrient broth, S5: sterile (habitat mud consisting cattle manure + nutrient broth + yeast) and S6: tap water.
View Article and Find Full Text PDFCaregivers' Perspectives on Discussions of Medical Treatment Preferences for People Living with Dementia Are Associated with Their Dementia Health Literacy and the Caregiving Relationship.
Gerontologist
January 2025
Department of Health & Community Systems, School of Nursing, University of Pittsburgh, Pittsburgh, PA, USA.
Background And Objectives: People living with dementia experience progressive functional decline and increased dependence on caregivers. This study examined the influence of caregivers' dementia health literacy on perceptions of medical care preferences and advanced care planning (ACP) in people living with dementia.
Research Design And Methods: This analysis used data from a cross-sectional survey, "Care Planning for Individuals with Dementia", administered nationwide by Alzheimer's Disease Centers.
Environmental desiccation stress induces viable but non culturable state in Neisseria meningitidis.
Arch Microbiol
January 2025
Symbiosis School of Biological Sciences, Symbiosis International (Deemed University)Lavale, Pune, Maharashtra, 412115, India.
Environmental factors play a crucial role in bacterial virulence. During transmission, in a non-host environment bacteria are exposed to various environmental stress which could alter bacterial physiology and virulence. N.
View Article and Find Full Text PDFQuantitative Profiling pH Heterogeneity of Acidic Endolysosomal Compartments using Fluorescence Lifetime Imaging Microscopy.
Mol Biol Cell
January 2025
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147 USA.
The endo-lysosomal system plays a crucial role in maintaining cellular homeostasis and promoting organism fitness. The pH of its acidic compartments is a crucial parameter for proper function, and it is dynamically influenced by both intracellular and environmental factors. Here, we present a method based on fluorescence lifetime imaging microscopy (FLIM) for quantitatively analyzing the pH profiles of acidic endolysosomal compartments in diverse types of primary mammalian cells and in live organism .
View Article and Find Full Text PDFPEBP1 amplifies mitochondrial dysfunction-induced integrated stress response.
Elife
January 2025
Centre for Cellular Biology and Signalling, Zhejiang University-University of Edinburgh (ZJU-UoE) Institute, Haining, China.
Mitochondrial dysfunction is involved in numerous diseases and the aging process. The integrated stress response (ISR) serves as a critical adaptation mechanism to a variety of stresses, including those originating from mitochondria. By utilizing mass spectrometry-based cellular thermal shift assay (MS-CETSA), we uncovered that phosphatidylethanolamine-binding protein 1 (PEBP1), also known as Raf kinase inhibitory protein (RKIP), is thermally stabilized by stresses which induce mitochondrial ISR.
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