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Significance: Protoporphyrin IX (PpIX) delayed fluorescence (DF) is inversely related to the oxygen present in tissues and has potential as a novel biomarker for surgical guidance and real-time tissue metabolism assessment. Despite the unique promise of this technique, its successful clinical translation is limited by the low intensity emitted.

Aim: We developed a systematic study of ways to increase the PpIX DF signal through acquisition sampling changes, allowing optimized imaging at video rates.

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Background: Elemental analysis of teeth allows for exposure assessment during critical windows of development and is increasingly used to link early life exposures and health. The measurement of inorganic elements in teeth is challenging; laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is the most widely used technique.

Objective: Both synchrotron x-ray fluorescence (SXRF) and LA-ICP-MS have the capability to measure elemental distributions in teeth with each having distinct advantages and disadvantages.

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Performance of four thermophilic bacteria for primary sludge hydrolysis: Sludge disintegration and hydrolase activities.

Bioresour Technol

January 2025

College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address:

Thermophilic bacteria (TB) pretreatment is an efficient and environmentally friendly way for accelerating sludge hydrolysis. In this study, a complete comparison of the hydrolysis performance of Bacillus sp. AT07-1 (X1), Parageobacillus toebii X2 (X2), Geobacillus kaustophilus X3 (X3) and Parageobacillus toebii R-35642 (X4) was performed.

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The anaerobic bacterium Clostridium cellulovorans is a promising candidate for the sustainable production of biofuels and platform chemicals due to its cellulolytic properties. However, the genomic engineering of the species is hampered because of its poor genetic accessibility and the lack of genetic tools. To overcome this limitation, a protocol for triparental conjugation was established that enables the reliable transfer of vectors for markerless chromosomal modification into C.

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Characterization and application of fluorescent hydrogel films with superior mechanical properties in detecting iron(Ⅲ) ions and ferroptosis in oral cancer.

Front Bioeng Biotechnol

January 2025

Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China.

A one-step hydrothermal method was applied to prepare carbon dots (CDs) with superior fluorescence properties using chitosan as a carbon source. The as-prepared carbon dots were then grafted onto a sodium alginate-gelatin hydrogel film to form a fluorescent hydrogel film (FHGF), emitting at 450 nm under excitation of 350 nm light. In comparison to the CDs, the fluorescence intensity of this film was maintained over 90.

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