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Cultured human sole-derived keratinocyte grafts re-express site-specific differentiation after transplantation.
Differentiation
November 1998
Department of Pathology, Massachusetts General Hospital 02114, USA.
Cultured epithelial autografts (CEA) derived from sole skin were transplanted to full-thickness wounds excised to muscle fascia over a variety of diverse body sites in 12 pediatric patients treated for acute burns or giant congenital nevi. The skin regenerated from the grafts was biopsied from 7 days to 6 years after grafting. The resultant epidermal phenotype was analyzed histologically and by immunohistochemical localization of keratin 9 (K9) as objective evidence of sole-type site-specific differentiation.
View Article and Find Full Text PDFDifferential expression of epithelial sodium channel subunit mRNAs in rat skin.
J Cell Sci
February 1996
INSERM U246, Institut Fédératif de Recherche Cellules Epithéliales, Paris, France.
Three subunits (alpha, beta, gamma) of the amiloride-sensitive epithelial sodium channel have been recently characterized. The channel subunits have significant homologies with the Caenorhabditis elegans mec-4, mec-10 and deg-1 genes, which are involved in control of cell volume and mecanotransduction. These subunits are coexpressed at equivalent levels in the renal collecting duct and the distal colon epithelium which are high resistance sodium transporting epithelia.
View Article and Find Full Text PDFHuman Merkel cell regeneration in skin derived from cultured keratinocyte grafts.
Lab Invest
August 1990
Department of Pathology, Harvard Medical School, Shriners Burns Institute of Boston, Massachusetts.
Human Merkel cell regeneration in epidermis derived from cultured keratinocyte autografts was studied from 6 days to 6 years after transplantation. Cultured keratinocyte sheets derived from skin of the sole, axilla, groin, or scalp were transplanted to full-thickness wounds in 20 pediatric patients treated for massive burns or giant congenital nevi. Normal age- and site-matched skin as well as meshed split-thickness autografts from the same patients served as controls.
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