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One-step transformation of CO to methane by Escherichia coli with a synthetic biomethanation module.
Biochem Biophys Res Commun
January 2025
Biofuels Institute, School of Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, China; School of Emergency Management, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou, 215009, China. Electronic address:
The biomethanation process is widely recognized as a significant approach to mitigating carbon dioxide emissions while simultaneously generating methane. However, only a few microorganisms that required intricate culturing conditions were identified for biomethanation. Here, Escherichia coli that featured easy cultivation and versatile chassis was genetically modified for biomethanation for the first time.
View Article and Find Full Text PDFExploring 1,2,3-triazole-Schiff's base hybrids as innovative EGFR inhibitors for the treatment of breast cancer: In vitro and in silico study.
Bioorg Chem
January 2025
Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. Electronic address:
EGFR inhibitors are a class of targeted therapies utilized in the management of certain tumor kinds such as NSCLC and breast cancer. Series of 1,2,3-triazole-Schiff's base hybrids were designed, synthesized, and estimated for their antitumor effect toward breast cancer cells, MCF-7 and MDA-MB-231. The safety and selectivity of the new compounds were tested using normal cell (WI-38).
View Article and Find Full Text PDFA novel LC-MS/MS assay for low concentrations of creatinine in sweat and saliva to validate biosensors for continuous monitoring of renal function.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven 5623 EJ, The Netherlands; Department of Biomedical Engineering, Chemical Biology, Eindhoven University of Technology, Groene Loper 3, Eindhoven 5612 AE, The Netherlands.
Monitoring of kidney function traditionally relies on plasma creatinine concentrations, necessitating invasive blood draws. Non-invasively obtainable biofluids, such as sweat and saliva, present a patient-friendly alternative with potential for continuous monitoring. This study focusses on developing and validating a novel Liquid Chromatography- tandem Mass Spectrometry (LC-MS/MS) assay as a reference test for measuring low creatinine concentrations in sweat and saliva.
View Article and Find Full Text PDFAcidobacteria as the dominant microorganism on the nitrogen-removal wastewater treatment with a low chemical oxygen demand/ammonium nitrogen ratio in biological contact oxidation reactor.
J Environ Manage
January 2025
College of Eco-environmental Technology, Guangdong Industry Polytechnic University, Guangzhou, 510300, China.
Nitrogen-removal promotion is a significant problem when biological nitrogen removal is used to treat ammonium nitrogen (NH-N) wastewater with a low chemical oxygen demand (COD)/NH-N (C/N) ratio. In this work, the biological nitrogen removal capacity of the biological contact oxidation reactor (BCOR) system was enhanced through the enrichment of Acidobacteria. The system was successfully started from Day 1 to Day 50 and stably operated through temperature, pH, and dissolved oxygen (DO) regulation from Day 51 to Day 254.
View Article and Find Full Text PDFComparative study of Idesia polycarpa Maxim cake meal polysaccharides: Conventional versus innovative extraction methods and their impact on structural features, emulsifying, antiglycation, and hypoglycemic properties.
Food Chem
January 2025
College of Food Science and Engineering, Guiyang University, Guiyang, Guizhou 550005, PR China; Engineering Technology Research Center for Processing and Comprehensive Utilization of Idesia polycarpa, National Forestry and Grassland Administration of the People's Republic of China, Guiyang, Guizhou 550005, PR China. Electronic address:
Idesia polycarpa Maxim (IPM) cake meal, a major by-product of oil extraction, is often discarded in large quantities, resulting in considerable waste. This study explored the extraction of IPM polysaccharides (IPMPs) from cake meal using the innovative ultrasonic-assisted three-phase partitioning (UTPP) method, in comparison with conventional techniques, including acid, medium-temperature alkali, chelating agent, and enzyme extraction methods. The IPMP-UT prepared via UTPP method achieved superior extraction efficiency (10.
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