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A Modified Novel Validated High-Throughput Hemagglutinin Inhibition Assay Using Recombinant Virus-like Particles and Human Red Blood Cells for the Objective Evaluation of Recombinant Hemagglutinin Nanoparticle Seasonal Influenza Vaccine.
Microorganisms
November 2024
Clinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Currently available seasonal influenza vaccines confer variable protection due to antigenic changes resulting from the accumulation of diverse mutations. The analysis of new seasonal influenza vaccines is challenging in part due to the limitations of the traditional hemagglutination inhibition (HAI) assay with A/H3N2 strains. An improved and objective novel HAI assay was developed with recombinant virus-like particles (VLPs) and an egg-derived virus as agglutinins, the oseltamivir treatment of VLPs, human red blood cells, and using an automated image reader-based analysis of hemagglutination.
View Article and Find Full Text PDFCommercial Day-Old Chicks in Nigeria Are Potential Reservoirs of Colistin- and Tigecycline-Resistant Potentially Pathogenic .
Antibiotics (Basel)
November 2024
Department of Livestock and Pasture Science, University of Fort Hare, Alice 5700, South Africa.
Frequent use of colistin (COL) and tetracyclines in the Nigerian poultry sector potentially triggers bacterial resistance against COL and tigecycline (TIG), which are last-line antibiotics used to treat multidrug-resistant infections. This study aimed to isolate COL- and TIG-resistant . from commercial day-old chicks distributed to poultry farmers in Nsukka Southeastern Nigeria, assess the production of extended-spectrum β-lactamase (ESBL) and carbapenemase by the isolates, and establish their pathogenic potentials.
View Article and Find Full Text PDFArticle Synopsis
- A recent outbreak of H5 avian influenza in cattle has spread across the U.S., affecting not only birds but also other mammals, including humans, with mostly mild symptoms reported in infected individuals.
- Research shows that mice with immunity to previous influenza strains were protected against severe H5N1 infections, and similarly, mice vaccinated with a live-attenuated influenza vaccine exhibited good protection against lethal H5N1 challenges.
- Ferrets with mixed immunity from vaccination or previous infections were also protected against a variant of the H5N1 virus, suggesting that factors beyond just antibody response, like T cell memory, might play a crucial role in providing protection against this virus.
Voltammetric-based immunosensing of Newcastle disease virus on polyethylene glycol-containing self-assembled monolayer modified gold electrode.
Anal Biochem
February 2025
Nanobiotechnology Research Group, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia; Laboratory of Virology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia; Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia. Electronic address:
Article Synopsis
- A voltammetric immunosensor was developed for detecting Newcastle disease virus (NDV) using a polyclonal antibody (anti-NDV) as a bioreceptor, which was immobilized on a PEG-containing self-assembled monolayer on a gold electrode.
- The sensor demonstrated a limit of detection of 1.50 HA μL and was characterized electrochemically using differential pulse voltammetry, showing a decrease in current as NDV concentration increased.
- The approach was shown to be specific to NDV with no significant changes in the presence of negative controls, making it a simple and efficient alternative to conventional NDV detection methods.
Safety and Immunogenicity Study of a Bivalent Vaccine for Combined Prophylaxis of COVID-19 and Influenza in Non-Human Primates.
Vaccines (Basel)
September 2024
Institute of Experimental Medicine, Saint-Petersburg 197022, Russia.
Background: Influenza and SARS-CoV-2 viruses are two highly variable pathogens. We have developed a candidate bivalent live vaccine based on the strain of licensed A/Leningrad/17-based cold-adapted live attenuated influenza vaccine (LAIV) of H3N2 subtype, which expressed SARS-CoV-2 immunogenic T-cell epitopes. A cassette encoding fragments of S and N proteins of SARS-CoV-2 was inserted into the influenza NA gene using the P2A autocleavage site.
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