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Corneal Endothelial Microscopy: Does a Manual Recognition of the Endothelial Cells Help the Morphometric Analysis Compared to a Fully Automatic Approach?
Vision (Basel)
October 2024
Department of Materials Sciences, University of Milano-Bicocca, 20125 Milan, Italy.
This study investigated whether manual integration in the recognition of the endothelial cells produces different outcomes of morphometric parameters compared to a fully automatic approach. Eight hundred and ninety endothelial images, originally acquired by the Perseus Specular Microscope (CSO, Florence, Italy), from seven positions of right and left corneas were selected from the database of the Research Centre in Optics and Optometry at the University of Milano-Bicocca. For each image selected, two procedures of cell identification were performed by the Perseus: an automatic identification and a manual-integrated procedure to add potential additional cells with the available editing tool.
View Article and Find Full Text PDFTissue-specific TCF4 triplet repeat instability revealed by optical genome mapping.
EBioMedicine
October 2024
UCL Institute of Ophthalmology, London, UK; Moorfields Eye Hospital, London, UK. Electronic address:
Background: Fuchs endothelial corneal dystrophy (FECD) is the most common repeat-mediated disease in humans. It exclusively affects corneal endothelial cells (CECs), with ≤81% of cases associated with an intronic TCF4 triplet repeat (CTG18.1).
View Article and Find Full Text PDFLight conditions and the evolution of the visual system in birds.
Evolution
July 2024
Centre for Ecological Research and Forestry Applications (CREAF), Spanish National Research Council (CSIC), Catalonia, Spain.
Despite vision being an essential sense for many animals, the intuitively appealing notion that the visual system has been shaped by environmental light conditions is backed by insufficient evidence. Based on a comprehensive phylogenetic comparative analysis of birds, we investigate if exposure to different light conditions might have triggered evolutionary divergence in the visual system through pressures on light sensitivity, visual acuity, and neural processing capacity. Our analyses suggest that birds that have adopted nocturnal habits evolved eyes with larger corneal diameters and, to a lesser extent, longer axial length than diurnal species.
View Article and Find Full Text PDFIn vivo confocal microscopy findings after Descemet stripping only with and without topical ripasudil supplementation in Fuchs endothelial dystrophy.
Can J Ophthalmol
December 2024
Discipline of Ophthalmology, Sydney Medical School, University of Sydney, Sydney, Australia; Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC.
Objective: To report corneal epithelial and corneal endothelial cell (CEC) changes following Descemet stripping only (DSO) with and without topical ripasudil using in vivo confocal microscopy (IVCM).
Methods: Prospective interventional case series of patients who underwent DSO for Fuchs endothelial dystrophy with or without postoperative topical ripasudil (4%, 6 times per day). Patients underwent IVCM (ConfoScan 3; NIDEK Technologies, Padova, Italy) at baseline, monthly until corneal clearance, and then every 6 months.
Oxygen-supplemented and topography-guided epithelium-on corneal crosslinking with pulsed irradiation for progressive keratoconus.
J Cataract Refract Surg
March 2024
From the Queensland Eye Institute, Brisbane, Queensland, Australia (Cronin, Gunn); Medical Affairs, Burlington, Massachusetts (Chang); Cornea Service, Wills Eye Hospital, Philadelphia, Pennsylvania (Chang).
Purpose: To investigate the effects of customized topography-guided epithelium-on crosslinking (epi-on CXL) with oxygen supplementation on procedural efficacy and corrected distance visual acuity (CDVA) in patients with progressive keratoconus (KC) at 1 year.
Setting: Private eye clinic, Brisbane, Australia.
Design: Retrospective, single-center, nonrandomized case series.
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