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Probing the impact of commercial cryoprotectants and freezing technique on the motility of human spermatozoa cryopreserved onto extremely water-repellent soot-coated surfaces.
Cryobiology
January 2025
Specialized Surgical Hospital "Doctor Malinov", 46, Gotse Delchev blvd., 1860, Sofia, Bulgaria.
The cryopreservation of human spermatozoa is an integral part of cryobiology, aiming to support the in-vitro fertilization. The latter relies on the availability of as much as possible reproductively active spermatozoa, whose number after thawing decreases due to the accompanied freezing injury and the cytotoxicity of cryoprotectants. An innovative option to circumvent these obstacles is to make the freezing interface non-wettable, by coating it with rapeseed oil soot possessing intrinsic cryoprotective properties, delaying the ice formation and possibly providing identical rates of intracellular dehydration and extracellular crystallization.
View Article and Find Full Text PDF[Genomic Characterization of SARS-CoV-2 Isolates Obtained from Antalya, Türkiye].
Mikrobiyol Bul
October 2024
The University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Division of Clinical Virology, Groningen, Netherlands.
As the number of coronavirus diseases-2019 (COVID-19) cases have decreased and measures have started to be implemented at an individual level rather than in the form of social restrictions, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) still maintains its importance and has already taken its place in the spectrum of agents investigated in multiplex molecular test panels for respiratory tract infections in routine diagnostic use. In this study, we aimed to present mutation analysis and clade distribution of whole genome sequences from randomly selected samples that tested positive with SARS-CoV-2 specific real-time reverse transcription polymerase chain reaction (rRT-PCR) test at different periods of the pandemic in our laboratory with a commercial easy-to-use kit designed for next-generation sequencing systems. A total of 84 nasopharyngeal/oropharyngeal swab samples of COVID-19 suspected patients which were sent for routine diagnosis to the medical microbiology laboratory and detected as SARSCoV-2 RNA positive with rRT-PCR were randomly selected from different periods for sequence analysis.
View Article and Find Full Text PDFBiomechanics of Negative-Pressure-Assisted Liposuction and Their Influence on Fat Regeneration.
Tissue Eng Part B Rev
November 2024
Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Shaanxi, China.
Autologous fat grafting has been widely adopted in cosmetic and reconstructive procedures recently. With the emerging of negative-pressure-assisted liposuction system, the harvesting process of fat grafting is more standardized, controllable, and efficient. Each component in the system could influence the biomechanical environment of lipoaspirate.
View Article and Find Full Text PDFReward Bases: A simple mechanism for adaptive acquisition of multiple reward types.
PLoS Comput Biol
November 2024
MRC Brain Network Dynamics Unit, University of Oxford, Oxford, United Kingdom.
Animals can adapt their preferences for different types of reward according to physiological state, such as hunger or thirst. To explain this ability, we employ a simple multi-objective reinforcement learning model that learns multiple values according to different reward dimensions such as food or water. We show that by weighting these learned values according to the current needs, behaviour may be flexibly adapted to present preferences.
View Article and Find Full Text PDFLight-field particle imaging velocimetry (LF-PIV) is widely used in large-scale flow field measurement scenarios due to its instant 3D imaging capability. However, conventional LF-PIV systems suffer low axial resolution and thereby have limited application in high-resolution and volumetric velocity measurements. Here, we report the use of synthetic color-and-depth-encoded (sCade) illumination to improve the axial resolution of LF-PIV.
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